Cargando…

Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP

BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonel...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheraghchi, Narges, Khaki, Pejvak, Moradi Bidhendi, Soheila, Sabokbar, Azar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255383/
https://www.ncbi.nlm.nih.gov/pubmed/25485068
http://dx.doi.org/10.5812/jjm.19135
_version_ 1782347426501230592
author Cheraghchi, Narges
Khaki, Pejvak
Moradi Bidhendi, Soheila
Sabokbar, Azar
author_facet Cheraghchi, Narges
Khaki, Pejvak
Moradi Bidhendi, Soheila
Sabokbar, Azar
author_sort Cheraghchi, Narges
collection PubMed
description BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES: The aim of this study was to identify S. gallinarum and S. pullorum by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MATERIALS AND METHODS: In this study, 13 samples of Salmonella, isolated from local poultry, were obtained from Razi Type Culture Collection (RTCC). For the PCR-RFLP method based on the fliC gene, extracted DNA was used as a template for amplifying of the fliC gene (197bp) using specific primers. PCR products were subjected to digestion using Hinp1I restriction endonuclease. RESULTS: For the PCR, 197 bp fliC fragment was amplified from all 13 isolates. Ten out of 13 were S. gallinarum and the other three were S. pullorum. As part of the PCR-RFLP, two fragments were obtained (82 bp and 115 bp) for all S. gallinarum, whereas no digestion was observed in S. pullorum, and 197 bp fragment was seen. CONCLUSIONS: PCR-RFLP with fliC gene and Hinp1I endonuclease were successfully applied to differentiate the two biotypes. The results suggested that this technique could be effective in detecting S. gallinarum and S. pullorum.
format Online
Article
Text
id pubmed-4255383
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Kowsar
record_format MEDLINE/PubMed
spelling pubmed-42553832014-12-05 Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP Cheraghchi, Narges Khaki, Pejvak Moradi Bidhendi, Soheila Sabokbar, Azar Jundishapur J Microbiol Research Article BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES: The aim of this study was to identify S. gallinarum and S. pullorum by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MATERIALS AND METHODS: In this study, 13 samples of Salmonella, isolated from local poultry, were obtained from Razi Type Culture Collection (RTCC). For the PCR-RFLP method based on the fliC gene, extracted DNA was used as a template for amplifying of the fliC gene (197bp) using specific primers. PCR products were subjected to digestion using Hinp1I restriction endonuclease. RESULTS: For the PCR, 197 bp fliC fragment was amplified from all 13 isolates. Ten out of 13 were S. gallinarum and the other three were S. pullorum. As part of the PCR-RFLP, two fragments were obtained (82 bp and 115 bp) for all S. gallinarum, whereas no digestion was observed in S. pullorum, and 197 bp fragment was seen. CONCLUSIONS: PCR-RFLP with fliC gene and Hinp1I endonuclease were successfully applied to differentiate the two biotypes. The results suggested that this technique could be effective in detecting S. gallinarum and S. pullorum. Kowsar 2014-09-01 2014-09 /pmc/articles/PMC4255383/ /pubmed/25485068 http://dx.doi.org/10.5812/jjm.19135 Text en Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Cheraghchi, Narges
Khaki, Pejvak
Moradi Bidhendi, Soheila
Sabokbar, Azar
Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title_full Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title_fullStr Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title_full_unstemmed Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title_short Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
title_sort identification of isolated salmonella enterica serotype gallinarum biotype pullorum and gallinarum by pcr-rflp
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255383/
https://www.ncbi.nlm.nih.gov/pubmed/25485068
http://dx.doi.org/10.5812/jjm.19135
work_keys_str_mv AT cheraghchinarges identificationofisolatedsalmonellaentericaserotypegallinarumbiotypepullorumandgallinarumbypcrrflp
AT khakipejvak identificationofisolatedsalmonellaentericaserotypegallinarumbiotypepullorumandgallinarumbypcrrflp
AT moradibidhendisoheila identificationofisolatedsalmonellaentericaserotypegallinarumbiotypepullorumandgallinarumbypcrrflp
AT sabokbarazar identificationofisolatedsalmonellaentericaserotypegallinarumbiotypepullorumandgallinarumbypcrrflp