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Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP
BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255383/ https://www.ncbi.nlm.nih.gov/pubmed/25485068 http://dx.doi.org/10.5812/jjm.19135 |
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author | Cheraghchi, Narges Khaki, Pejvak Moradi Bidhendi, Soheila Sabokbar, Azar |
author_facet | Cheraghchi, Narges Khaki, Pejvak Moradi Bidhendi, Soheila Sabokbar, Azar |
author_sort | Cheraghchi, Narges |
collection | PubMed |
description | BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES: The aim of this study was to identify S. gallinarum and S. pullorum by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MATERIALS AND METHODS: In this study, 13 samples of Salmonella, isolated from local poultry, were obtained from Razi Type Culture Collection (RTCC). For the PCR-RFLP method based on the fliC gene, extracted DNA was used as a template for amplifying of the fliC gene (197bp) using specific primers. PCR products were subjected to digestion using Hinp1I restriction endonuclease. RESULTS: For the PCR, 197 bp fliC fragment was amplified from all 13 isolates. Ten out of 13 were S. gallinarum and the other three were S. pullorum. As part of the PCR-RFLP, two fragments were obtained (82 bp and 115 bp) for all S. gallinarum, whereas no digestion was observed in S. pullorum, and 197 bp fragment was seen. CONCLUSIONS: PCR-RFLP with fliC gene and Hinp1I endonuclease were successfully applied to differentiate the two biotypes. The results suggested that this technique could be effective in detecting S. gallinarum and S. pullorum. |
format | Online Article Text |
id | pubmed-4255383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-42553832014-12-05 Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP Cheraghchi, Narges Khaki, Pejvak Moradi Bidhendi, Soheila Sabokbar, Azar Jundishapur J Microbiol Research Article BACKGROUND: Salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. Among Salmonella spp., Salmonella gallinarum and Salmonella. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. OBJECTIVES: The aim of this study was to identify S. gallinarum and S. pullorum by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. MATERIALS AND METHODS: In this study, 13 samples of Salmonella, isolated from local poultry, were obtained from Razi Type Culture Collection (RTCC). For the PCR-RFLP method based on the fliC gene, extracted DNA was used as a template for amplifying of the fliC gene (197bp) using specific primers. PCR products were subjected to digestion using Hinp1I restriction endonuclease. RESULTS: For the PCR, 197 bp fliC fragment was amplified from all 13 isolates. Ten out of 13 were S. gallinarum and the other three were S. pullorum. As part of the PCR-RFLP, two fragments were obtained (82 bp and 115 bp) for all S. gallinarum, whereas no digestion was observed in S. pullorum, and 197 bp fragment was seen. CONCLUSIONS: PCR-RFLP with fliC gene and Hinp1I endonuclease were successfully applied to differentiate the two biotypes. The results suggested that this technique could be effective in detecting S. gallinarum and S. pullorum. Kowsar 2014-09-01 2014-09 /pmc/articles/PMC4255383/ /pubmed/25485068 http://dx.doi.org/10.5812/jjm.19135 Text en Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Cheraghchi, Narges Khaki, Pejvak Moradi Bidhendi, Soheila Sabokbar, Azar Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title | Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title_full | Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title_fullStr | Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title_full_unstemmed | Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title_short | Identification of Isolated Salmonella enterica Serotype gallinarum Biotype Pullorum and Gallinarum by PCR-RFLP |
title_sort | identification of isolated salmonella enterica serotype gallinarum biotype pullorum and gallinarum by pcr-rflp |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255383/ https://www.ncbi.nlm.nih.gov/pubmed/25485068 http://dx.doi.org/10.5812/jjm.19135 |
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