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Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats

Granulocyte-colony stimulating factor (G-CSF) and bone marrow derived mononuclear cells (BM-MNCs) have both been shown to improve functional outcome following experimental stroke. These effects are associated with increased angiogenesis and neurogenesis. In the present study, we aimed to determine s...

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Autores principales: Diederich, Kai, Schmidt, Antje, Beuker, Carolin, Strecker, Jan-Kolja, Wagner, Daniel-Christoph, Boltze, Johannes, Schäbitz, Wolf-Rüdiger, Minnerup, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255603/
https://www.ncbi.nlm.nih.gov/pubmed/25538562
http://dx.doi.org/10.3389/fncel.2014.00411
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author Diederich, Kai
Schmidt, Antje
Beuker, Carolin
Strecker, Jan-Kolja
Wagner, Daniel-Christoph
Boltze, Johannes
Schäbitz, Wolf-Rüdiger
Minnerup, Jens
author_facet Diederich, Kai
Schmidt, Antje
Beuker, Carolin
Strecker, Jan-Kolja
Wagner, Daniel-Christoph
Boltze, Johannes
Schäbitz, Wolf-Rüdiger
Minnerup, Jens
author_sort Diederich, Kai
collection PubMed
description Granulocyte-colony stimulating factor (G-CSF) and bone marrow derived mononuclear cells (BM-MNCs) have both been shown to improve functional outcome following experimental stroke. These effects are associated with increased angiogenesis and neurogenesis. In the present study, we aimed to determine synergistic effects of G-CSF and BM-NMC treatment on long-term structural and functional recovery after photothrombotic stroke. To model the etiology of stroke more closely, we used spontaneously hypertensive (SH) rats in our experiment. Bone marrow derived mononuclear cells transplantation was initiated 1 h after the onset of photothrombotic stroke. Repeated G-CSF treatment commenced immediately after BM-MNC treatment followed by daily injections for five consecutive days. The primary endpoint was functional outcome after ischemia. Secondary endpoints included analysis of neurogenesis and angiogenesis as well as determination of infarct size. Granulocyte-colony stimulating factor treated rats, either in combination with BM-MNC or alone showed improved somatosensory but not gross motor function following ischemia. No beneficial effect of BM-MNC monotherapy was found. Infarct volumes were comparable in all groups. In contrast to previous studies, which used healthy animals, post-stroke neurogenesis and angiogenesis were not enhanced by G-CSF. In conclusion, the combination of G-CSF and BM-MNC was not more effective than G-CSF alone. The reduced efficacy of G-CSF treatment and the absence of any beneficial effect of BM-MNC transplantation might be attributed to hypertension-related morbidity.
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spelling pubmed-42556032014-12-23 Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats Diederich, Kai Schmidt, Antje Beuker, Carolin Strecker, Jan-Kolja Wagner, Daniel-Christoph Boltze, Johannes Schäbitz, Wolf-Rüdiger Minnerup, Jens Front Cell Neurosci Neuroscience Granulocyte-colony stimulating factor (G-CSF) and bone marrow derived mononuclear cells (BM-MNCs) have both been shown to improve functional outcome following experimental stroke. These effects are associated with increased angiogenesis and neurogenesis. In the present study, we aimed to determine synergistic effects of G-CSF and BM-NMC treatment on long-term structural and functional recovery after photothrombotic stroke. To model the etiology of stroke more closely, we used spontaneously hypertensive (SH) rats in our experiment. Bone marrow derived mononuclear cells transplantation was initiated 1 h after the onset of photothrombotic stroke. Repeated G-CSF treatment commenced immediately after BM-MNC treatment followed by daily injections for five consecutive days. The primary endpoint was functional outcome after ischemia. Secondary endpoints included analysis of neurogenesis and angiogenesis as well as determination of infarct size. Granulocyte-colony stimulating factor treated rats, either in combination with BM-MNC or alone showed improved somatosensory but not gross motor function following ischemia. No beneficial effect of BM-MNC monotherapy was found. Infarct volumes were comparable in all groups. In contrast to previous studies, which used healthy animals, post-stroke neurogenesis and angiogenesis were not enhanced by G-CSF. In conclusion, the combination of G-CSF and BM-MNC was not more effective than G-CSF alone. The reduced efficacy of G-CSF treatment and the absence of any beneficial effect of BM-MNC transplantation might be attributed to hypertension-related morbidity. Frontiers Media S.A. 2014-12-04 /pmc/articles/PMC4255603/ /pubmed/25538562 http://dx.doi.org/10.3389/fncel.2014.00411 Text en Copyright © 2014 Diederich, Schmidt, Beuker, Strecker, Wagner, Boltze, Schäbitz and Minnerup. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Diederich, Kai
Schmidt, Antje
Beuker, Carolin
Strecker, Jan-Kolja
Wagner, Daniel-Christoph
Boltze, Johannes
Schäbitz, Wolf-Rüdiger
Minnerup, Jens
Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title_full Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title_fullStr Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title_full_unstemmed Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title_short Granulocyte colony-stimulating factor (G-CSF) treatment in combination with transplantation of bone marrow cells is not superior to G-CSF treatment alone after cortical stroke in spontaneously hypertensive rats
title_sort granulocyte colony-stimulating factor (g-csf) treatment in combination with transplantation of bone marrow cells is not superior to g-csf treatment alone after cortical stroke in spontaneously hypertensive rats
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255603/
https://www.ncbi.nlm.nih.gov/pubmed/25538562
http://dx.doi.org/10.3389/fncel.2014.00411
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