First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex

Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes w...

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Autores principales: Pothirat, Thatawan, Tantiworawit, Adisak, Chaiwarith, Romanee, Jariyapan, Narissara, Wannasan, Anchalee, Siriyasatien, Padet, Supparatpinyo, Khuanchai, Bates, Michelle D., Kwakye-Nuako, Godwin, Bates, Paul A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256172/
https://www.ncbi.nlm.nih.gov/pubmed/25474647
http://dx.doi.org/10.1371/journal.pntd.0003339
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author Pothirat, Thatawan
Tantiworawit, Adisak
Chaiwarith, Romanee
Jariyapan, Narissara
Wannasan, Anchalee
Siriyasatien, Padet
Supparatpinyo, Khuanchai
Bates, Michelle D.
Kwakye-Nuako, Godwin
Bates, Paul A.
author_facet Pothirat, Thatawan
Tantiworawit, Adisak
Chaiwarith, Romanee
Jariyapan, Narissara
Wannasan, Anchalee
Siriyasatien, Padet
Supparatpinyo, Khuanchai
Bates, Michelle D.
Kwakye-Nuako, Godwin
Bates, Paul A.
author_sort Pothirat, Thatawan
collection PubMed
description Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as “L. siamensis” also appear to be L. martiniquensis.
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spelling pubmed-42561722014-12-11 First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex Pothirat, Thatawan Tantiworawit, Adisak Chaiwarith, Romanee Jariyapan, Narissara Wannasan, Anchalee Siriyasatien, Padet Supparatpinyo, Khuanchai Bates, Michelle D. Kwakye-Nuako, Godwin Bates, Paul A. PLoS Negl Trop Dis Research Article Since 1996, there have been several case reports of autochthonous visceral leishmaniasis in Thailand. Here we report a case in a 52-year-old Thai male from northern Thailand, who presented with subacute fever, huge splenomegaly and pancytopenia. Bone marrow aspiration revealed numerous amastigotes within macrophages. Isolation of Leishmania LSCM1 into culture and DNA sequence analysis (ribosomal RNA ITS-1 and large subunit of RNA polymerase II) revealed the parasites to be members of the Leishmania enriettii complex, and apparently identical to L. martiniquensis previously reported from the Caribbean island of Martinique. This is the first report of visceral leishmaniasis caused by L. martiniquensis from the region. Moreover, the majority of parasites previously identified as “L. siamensis” also appear to be L. martiniquensis. Public Library of Science 2014-12-04 /pmc/articles/PMC4256172/ /pubmed/25474647 http://dx.doi.org/10.1371/journal.pntd.0003339 Text en © 2014 Pothirat et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pothirat, Thatawan
Tantiworawit, Adisak
Chaiwarith, Romanee
Jariyapan, Narissara
Wannasan, Anchalee
Siriyasatien, Padet
Supparatpinyo, Khuanchai
Bates, Michelle D.
Kwakye-Nuako, Godwin
Bates, Paul A.
First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title_full First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title_fullStr First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title_full_unstemmed First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title_short First Isolation of Leishmania from Northern Thailand: Case Report, Identification as Leishmania martiniquensis and Phylogenetic Position within the Leishmania enriettii Complex
title_sort first isolation of leishmania from northern thailand: case report, identification as leishmania martiniquensis and phylogenetic position within the leishmania enriettii complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256172/
https://www.ncbi.nlm.nih.gov/pubmed/25474647
http://dx.doi.org/10.1371/journal.pntd.0003339
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