Cargando…

Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity

The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE)...

Descripción completa

Detalles Bibliográficos
Autores principales: Kingston, Diego, Novelli, Guido F, Cerrutti, Patricia, Recupero, Matias N, Blasco, Martin, Galvagno, Miguel A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256579/
https://www.ncbi.nlm.nih.gov/pubmed/25493192
http://dx.doi.org/10.1002/fsn3.128
_version_ 1782347606460989440
author Kingston, Diego
Novelli, Guido F
Cerrutti, Patricia
Recupero, Matias N
Blasco, Martin
Galvagno, Miguel A
author_facet Kingston, Diego
Novelli, Guido F
Cerrutti, Patricia
Recupero, Matias N
Blasco, Martin
Galvagno, Miguel A
author_sort Kingston, Diego
collection PubMed
description The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE) were proposed as a convenient C-energy source for the biomass production of the genetically engineered strain. Carbon and nitrogen sources, growth factors, and initial pH conditions were selected by classical methodology; thereafter, growth conditions optimization was performed using statistical designed experiments (DoEs). In the presence of (in g·L(−1)) 67.0 monosaccharides (glucose and fructose) from GRE, 5.0 (NH(4))(2)SO(4), and 10.0 sugar cane molasses (CMz), a yield of 20.0 g·L(−1) cell dry weight (CDW) was obtained aerobically after 60 h incubation at 28°C and pH 4.0. Applying a fed-batch strategy with methanol:sorbitol as the enzyme inducers, a chymosin production of 8.53 International Milk Clotting Units (IMCU) per mg protein was obtained in the supernatant. The results presented show that through a statistical design, a simple, cheap, and easy to prepare culture medium could be developed using two agroindustrial derivatives (GRE and CMz) to obtain a higher value added product.
format Online
Article
Text
id pubmed-4256579
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BlackWell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-42565792014-12-09 Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity Kingston, Diego Novelli, Guido F Cerrutti, Patricia Recupero, Matias N Blasco, Martin Galvagno, Miguel A Food Sci Nutr Original Research The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE) were proposed as a convenient C-energy source for the biomass production of the genetically engineered strain. Carbon and nitrogen sources, growth factors, and initial pH conditions were selected by classical methodology; thereafter, growth conditions optimization was performed using statistical designed experiments (DoEs). In the presence of (in g·L(−1)) 67.0 monosaccharides (glucose and fructose) from GRE, 5.0 (NH(4))(2)SO(4), and 10.0 sugar cane molasses (CMz), a yield of 20.0 g·L(−1) cell dry weight (CDW) was obtained aerobically after 60 h incubation at 28°C and pH 4.0. Applying a fed-batch strategy with methanol:sorbitol as the enzyme inducers, a chymosin production of 8.53 International Milk Clotting Units (IMCU) per mg protein was obtained in the supernatant. The results presented show that through a statistical design, a simple, cheap, and easy to prepare culture medium could be developed using two agroindustrial derivatives (GRE and CMz) to obtain a higher value added product. BlackWell Publishing Ltd 2014-11 2014-09-12 /pmc/articles/PMC4256579/ /pubmed/25493192 http://dx.doi.org/10.1002/fsn3.128 Text en © 2014 The Authors.Food Science & Nutrition published by Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Kingston, Diego
Novelli, Guido F
Cerrutti, Patricia
Recupero, Matias N
Blasco, Martin
Galvagno, Miguel A
Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title_full Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title_fullStr Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title_full_unstemmed Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title_short Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
title_sort use of grape pomaces to produce biomass of akomagataella pastoris strain expressing a bovine chymosin activity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256579/
https://www.ncbi.nlm.nih.gov/pubmed/25493192
http://dx.doi.org/10.1002/fsn3.128
work_keys_str_mv AT kingstondiego useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity
AT novelliguidof useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity
AT cerruttipatricia useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity
AT recuperomatiasn useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity
AT blascomartin useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity
AT galvagnomiguela useofgrapepomacestoproducebiomassofakomagataellapastorisstrainexpressingabovinechymosinactivity