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Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity
The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE)...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256579/ https://www.ncbi.nlm.nih.gov/pubmed/25493192 http://dx.doi.org/10.1002/fsn3.128 |
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author | Kingston, Diego Novelli, Guido F Cerrutti, Patricia Recupero, Matias N Blasco, Martin Galvagno, Miguel A |
author_facet | Kingston, Diego Novelli, Guido F Cerrutti, Patricia Recupero, Matias N Blasco, Martin Galvagno, Miguel A |
author_sort | Kingston, Diego |
collection | PubMed |
description | The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE) were proposed as a convenient C-energy source for the biomass production of the genetically engineered strain. Carbon and nitrogen sources, growth factors, and initial pH conditions were selected by classical methodology; thereafter, growth conditions optimization was performed using statistical designed experiments (DoEs). In the presence of (in g·L(−1)) 67.0 monosaccharides (glucose and fructose) from GRE, 5.0 (NH(4))(2)SO(4), and 10.0 sugar cane molasses (CMz), a yield of 20.0 g·L(−1) cell dry weight (CDW) was obtained aerobically after 60 h incubation at 28°C and pH 4.0. Applying a fed-batch strategy with methanol:sorbitol as the enzyme inducers, a chymosin production of 8.53 International Milk Clotting Units (IMCU) per mg protein was obtained in the supernatant. The results presented show that through a statistical design, a simple, cheap, and easy to prepare culture medium could be developed using two agroindustrial derivatives (GRE and CMz) to obtain a higher value added product. |
format | Online Article Text |
id | pubmed-4256579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42565792014-12-09 Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity Kingston, Diego Novelli, Guido F Cerrutti, Patricia Recupero, Matias N Blasco, Martin Galvagno, Miguel A Food Sci Nutr Original Research The use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol-inducible bovine chymosin has been expressed in our laboratory in the yeastKomagataella pastoris, and grape pomace extracts (GRE) were proposed as a convenient C-energy source for the biomass production of the genetically engineered strain. Carbon and nitrogen sources, growth factors, and initial pH conditions were selected by classical methodology; thereafter, growth conditions optimization was performed using statistical designed experiments (DoEs). In the presence of (in g·L(−1)) 67.0 monosaccharides (glucose and fructose) from GRE, 5.0 (NH(4))(2)SO(4), and 10.0 sugar cane molasses (CMz), a yield of 20.0 g·L(−1) cell dry weight (CDW) was obtained aerobically after 60 h incubation at 28°C and pH 4.0. Applying a fed-batch strategy with methanol:sorbitol as the enzyme inducers, a chymosin production of 8.53 International Milk Clotting Units (IMCU) per mg protein was obtained in the supernatant. The results presented show that through a statistical design, a simple, cheap, and easy to prepare culture medium could be developed using two agroindustrial derivatives (GRE and CMz) to obtain a higher value added product. BlackWell Publishing Ltd 2014-11 2014-09-12 /pmc/articles/PMC4256579/ /pubmed/25493192 http://dx.doi.org/10.1002/fsn3.128 Text en © 2014 The Authors.Food Science & Nutrition published by Wiley Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Kingston, Diego Novelli, Guido F Cerrutti, Patricia Recupero, Matias N Blasco, Martin Galvagno, Miguel A Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title | Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title_full | Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title_fullStr | Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title_full_unstemmed | Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title_short | Use of grape pomaces to produce biomass of aKomagataella pastoris strain expressing a bovine chymosin activity |
title_sort | use of grape pomaces to produce biomass of akomagataella pastoris strain expressing a bovine chymosin activity |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256579/ https://www.ncbi.nlm.nih.gov/pubmed/25493192 http://dx.doi.org/10.1002/fsn3.128 |
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