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Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina

OBJECTIVE: To describe an optimized surgical technique for feline vitrectomy which reduces bleeding and aids posterior gel clearance in order to facilitate stem cell delivery to the inner retina using cellular scaffolds. PROCEDURES: Three-port pars plana vitrectomies were performed in six-specific p...

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Autores principales: Jayaram, Hari, Becker, Silke, Eastlake, Karen, Jones, Megan F, Charteris, David G, Limb, G Astrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4258072/
https://www.ncbi.nlm.nih.gov/pubmed/24661435
http://dx.doi.org/10.1111/vop.12160
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author Jayaram, Hari
Becker, Silke
Eastlake, Karen
Jones, Megan F
Charteris, David G
Limb, G Astrid
author_facet Jayaram, Hari
Becker, Silke
Eastlake, Karen
Jones, Megan F
Charteris, David G
Limb, G Astrid
author_sort Jayaram, Hari
collection PubMed
description OBJECTIVE: To describe an optimized surgical technique for feline vitrectomy which reduces bleeding and aids posterior gel clearance in order to facilitate stem cell delivery to the inner retina using cellular scaffolds. PROCEDURES: Three-port pars plana vitrectomies were performed in six-specific pathogen-free domestic cats using an optimized surgical technique to improve access and minimize severe intraoperative bleeding. RESULTS: The surgical procedure was successfully completed in all six animals. Lens sparing vitrectomy resulted in peripheral lens touch in one of three animals but without cataract formation. Transient bleeding from sclerotomies, which was readily controlled, was seen in two of the six animals. No cases of vitreous hemorrhage, severe postoperative inflammation, retinal detachment, or endophthalmitis were observed during postoperative follow-up. CONCLUSIONS: Three-port pars plana vitrectomy can be performed successfully in the cat in a safe and controlled manner when the appropriate precautions are taken to minimize the risk of developing intraoperative hemorrhage. This technique may facilitate the use of feline models of inner retinal degeneration for the development of stem cell transplantation techniques using cellular scaffolds.
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spelling pubmed-42580722014-12-11 Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina Jayaram, Hari Becker, Silke Eastlake, Karen Jones, Megan F Charteris, David G Limb, G Astrid Vet Ophthalmol Brief Communications OBJECTIVE: To describe an optimized surgical technique for feline vitrectomy which reduces bleeding and aids posterior gel clearance in order to facilitate stem cell delivery to the inner retina using cellular scaffolds. PROCEDURES: Three-port pars plana vitrectomies were performed in six-specific pathogen-free domestic cats using an optimized surgical technique to improve access and minimize severe intraoperative bleeding. RESULTS: The surgical procedure was successfully completed in all six animals. Lens sparing vitrectomy resulted in peripheral lens touch in one of three animals but without cataract formation. Transient bleeding from sclerotomies, which was readily controlled, was seen in two of the six animals. No cases of vitreous hemorrhage, severe postoperative inflammation, retinal detachment, or endophthalmitis were observed during postoperative follow-up. CONCLUSIONS: Three-port pars plana vitrectomy can be performed successfully in the cat in a safe and controlled manner when the appropriate precautions are taken to minimize the risk of developing intraoperative hemorrhage. This technique may facilitate the use of feline models of inner retinal degeneration for the development of stem cell transplantation techniques using cellular scaffolds. BlackWell Publishing Ltd 2014-07 2014-03-24 /pmc/articles/PMC4258072/ /pubmed/24661435 http://dx.doi.org/10.1111/vop.12160 Text en © 2014 The Authors Veterinary Ophthalmology published by Wiley Periodicals, Inc. on behalf of American College of Veterinary Ophthalmologists http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communications
Jayaram, Hari
Becker, Silke
Eastlake, Karen
Jones, Megan F
Charteris, David G
Limb, G Astrid
Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title_full Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title_fullStr Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title_full_unstemmed Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title_short Optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
title_sort optimized feline vitrectomy technique for therapeutic stem cell delivery to the inner retina
topic Brief Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4258072/
https://www.ncbi.nlm.nih.gov/pubmed/24661435
http://dx.doi.org/10.1111/vop.12160
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