A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

A method is described for using plate lids to reduce evaporation in low-volume vapor-diffusion crystallization experiments. The plate lids contain apertures through which the protein and precipitants were added to different crystallization microplates (the reservoir was filled before fitting the lid...

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Detalles Bibliográficos
Autores principales: Zipper, Lauren E., Aristide, Xavier, Bishop, Dylan P., Joshi, Ishita, Kharzeev, Julia, Patel, Krishna B., Santiago, Brianna M., Joshi, Karan, Dorsinvil, Kahille, Sweet, Robert M., Soares, Alexei S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2014
Materias:
Laboratory Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259245/
https://www.ncbi.nlm.nih.gov/pubmed/25484231
http://dx.doi.org/10.1107/S2053230X14025126

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259245/
https://www.ncbi.nlm.nih.gov/pubmed/25484231
http://dx.doi.org/10.1107/S2053230X14025126

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