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Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors

Plant growth regulators, such as hormones and their respective biosynthesis inhibitors, are effective tools to elucidate the physiological function of phytohormones in plants. A problem of chemical treatments, however, is the potential for interaction of the active compound with the growth media sub...

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Autores principales: Best, Norman B., Hartwig, Thomas, Budka, Joshua S., Bishop, Brandon J., Brown, Elliot, Potluri, Devi P. V., Cooper, Bruce R., Premachandra, Gnanasiri S., Johnston, Cliff T., Schulz, Burkhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259294/
https://www.ncbi.nlm.nih.gov/pubmed/25485677
http://dx.doi.org/10.1371/journal.pone.0107689
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author Best, Norman B.
Hartwig, Thomas
Budka, Joshua S.
Bishop, Brandon J.
Brown, Elliot
Potluri, Devi P. V.
Cooper, Bruce R.
Premachandra, Gnanasiri S.
Johnston, Cliff T.
Schulz, Burkhard
author_facet Best, Norman B.
Hartwig, Thomas
Budka, Joshua S.
Bishop, Brandon J.
Brown, Elliot
Potluri, Devi P. V.
Cooper, Bruce R.
Premachandra, Gnanasiri S.
Johnston, Cliff T.
Schulz, Burkhard
author_sort Best, Norman B.
collection PubMed
description Plant growth regulators, such as hormones and their respective biosynthesis inhibitors, are effective tools to elucidate the physiological function of phytohormones in plants. A problem of chemical treatments, however, is the potential for interaction of the active compound with the growth media substrate. We studied the interaction and efficacy of propiconazole, a potent and specific inhibitor of brassinosteroid biosynthesis, with common soilless greenhouse growth media for rice, sorghum, and maize. Many of the tested growth media interacted with propiconazole reducing its efficacy up to a hundred fold. To determine the molecular interaction of inhibitors with media substrates, Fourier Transform Infrared Spectroscopy and sorption isotherm analysis was applied. While mica clay substrates absorbed up to 1.3 mg of propiconazole per g substrate, calcined clays bound up to 12 mg of propiconazole per g substrate. The efficacy of the gibberellic acid biosynthesis inhibitor, uniconazole, and the most active brassinosteroid, brassinolide, was impacted similarly by the respective substrates. Conversely, gibberellic acid showed no distinct growth response in different media. Our results suggest that the reduction in efficacy of propiconazole, uniconazole, and brassinolide in bioassays when grown in calcined clay is caused by hydrophobic interactions between the plant growth regulators and the growth media. This was further confirmed by experiments using methanol-water solvent mixes with higher hydrophobicity values, which reduce the interaction of propiconazole and calcined clay.
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spelling pubmed-42592942014-12-15 Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors Best, Norman B. Hartwig, Thomas Budka, Joshua S. Bishop, Brandon J. Brown, Elliot Potluri, Devi P. V. Cooper, Bruce R. Premachandra, Gnanasiri S. Johnston, Cliff T. Schulz, Burkhard PLoS One Research Article Plant growth regulators, such as hormones and their respective biosynthesis inhibitors, are effective tools to elucidate the physiological function of phytohormones in plants. A problem of chemical treatments, however, is the potential for interaction of the active compound with the growth media substrate. We studied the interaction and efficacy of propiconazole, a potent and specific inhibitor of brassinosteroid biosynthesis, with common soilless greenhouse growth media for rice, sorghum, and maize. Many of the tested growth media interacted with propiconazole reducing its efficacy up to a hundred fold. To determine the molecular interaction of inhibitors with media substrates, Fourier Transform Infrared Spectroscopy and sorption isotherm analysis was applied. While mica clay substrates absorbed up to 1.3 mg of propiconazole per g substrate, calcined clays bound up to 12 mg of propiconazole per g substrate. The efficacy of the gibberellic acid biosynthesis inhibitor, uniconazole, and the most active brassinosteroid, brassinolide, was impacted similarly by the respective substrates. Conversely, gibberellic acid showed no distinct growth response in different media. Our results suggest that the reduction in efficacy of propiconazole, uniconazole, and brassinolide in bioassays when grown in calcined clay is caused by hydrophobic interactions between the plant growth regulators and the growth media. This was further confirmed by experiments using methanol-water solvent mixes with higher hydrophobicity values, which reduce the interaction of propiconazole and calcined clay. Public Library of Science 2014-12-08 /pmc/articles/PMC4259294/ /pubmed/25485677 http://dx.doi.org/10.1371/journal.pone.0107689 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Best, Norman B.
Hartwig, Thomas
Budka, Joshua S.
Bishop, Brandon J.
Brown, Elliot
Potluri, Devi P. V.
Cooper, Bruce R.
Premachandra, Gnanasiri S.
Johnston, Cliff T.
Schulz, Burkhard
Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title_full Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title_fullStr Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title_full_unstemmed Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title_short Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors
title_sort soilless plant growth media influence the efficacy of phytohormones and phytohormone inhibitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259294/
https://www.ncbi.nlm.nih.gov/pubmed/25485677
http://dx.doi.org/10.1371/journal.pone.0107689
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