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In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts
Peri-prosthetic infections caused by multidrug resistant bacteria have become a serious problem in surgery and orthopedics. The aim is to introduce biomaterials that avoid implant-related infections caused by multiresistant bacteria. The efficacy of silver nanoparticles (AgNP) against a broad spectr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259364/ https://www.ncbi.nlm.nih.gov/pubmed/25485700 http://dx.doi.org/10.1371/journal.pone.0114740 |
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author | Pauksch, Linda Hartmann, Sonja Szalay, Gabor Alt, Volker Lips, Katrin S. |
author_facet | Pauksch, Linda Hartmann, Sonja Szalay, Gabor Alt, Volker Lips, Katrin S. |
author_sort | Pauksch, Linda |
collection | PubMed |
description | Peri-prosthetic infections caused by multidrug resistant bacteria have become a serious problem in surgery and orthopedics. The aim is to introduce biomaterials that avoid implant-related infections caused by multiresistant bacteria. The efficacy of silver nanoparticles (AgNP) against a broad spectrum of bacteria and against multiresistant pathogens has been repeatedly described. In the present study polymethylmethacrylate (PMMA) bone cement functionalized with AgNP and/or gentamicin were tested regarding their biocompatibility with bone forming cells. Therefore, influences on viability, cell number and differentiation of primary human mesenchymal stem cells (MSCs) and MSCs cultured in osteogenic differentiation media (MSC-OM) caused by the implant materials were studied. Furthermore, the growth behavior and the morphology of the cells on the testing material were observed. Finally, we examined the induction of cell stress, regarding antioxidative defense and endoplasmatic reticulum stress. We demonstrated similar cytocompatibility of PMMA loaded with AgNP compared to plain PMMA or PMMA loaded with gentamicin. There was no decrease in cell number, viability and osteogenic differentiation and no induction of cell stress for all three PMMA variants after 21 days. Addition of gentamicin to AgNP-loaded PMMA led to a slight decrease in osteogenic differentiation. Also an increase in cell stress was detectable for PMMA loaded with gentamicin and AgNP. In conclusion, supplementation of PMMA bone cement with gentamicin, AgNP, and both results in bone implants with an antibacterial potency and suitable cytocompatibility in MSCs and MSC-OM. |
format | Online Article Text |
id | pubmed-4259364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42593642014-12-15 In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts Pauksch, Linda Hartmann, Sonja Szalay, Gabor Alt, Volker Lips, Katrin S. PLoS One Research Article Peri-prosthetic infections caused by multidrug resistant bacteria have become a serious problem in surgery and orthopedics. The aim is to introduce biomaterials that avoid implant-related infections caused by multiresistant bacteria. The efficacy of silver nanoparticles (AgNP) against a broad spectrum of bacteria and against multiresistant pathogens has been repeatedly described. In the present study polymethylmethacrylate (PMMA) bone cement functionalized with AgNP and/or gentamicin were tested regarding their biocompatibility with bone forming cells. Therefore, influences on viability, cell number and differentiation of primary human mesenchymal stem cells (MSCs) and MSCs cultured in osteogenic differentiation media (MSC-OM) caused by the implant materials were studied. Furthermore, the growth behavior and the morphology of the cells on the testing material were observed. Finally, we examined the induction of cell stress, regarding antioxidative defense and endoplasmatic reticulum stress. We demonstrated similar cytocompatibility of PMMA loaded with AgNP compared to plain PMMA or PMMA loaded with gentamicin. There was no decrease in cell number, viability and osteogenic differentiation and no induction of cell stress for all three PMMA variants after 21 days. Addition of gentamicin to AgNP-loaded PMMA led to a slight decrease in osteogenic differentiation. Also an increase in cell stress was detectable for PMMA loaded with gentamicin and AgNP. In conclusion, supplementation of PMMA bone cement with gentamicin, AgNP, and both results in bone implants with an antibacterial potency and suitable cytocompatibility in MSCs and MSC-OM. Public Library of Science 2014-12-08 /pmc/articles/PMC4259364/ /pubmed/25485700 http://dx.doi.org/10.1371/journal.pone.0114740 Text en © 2014 Pauksch et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pauksch, Linda Hartmann, Sonja Szalay, Gabor Alt, Volker Lips, Katrin S. In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title | In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title_full | In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title_fullStr | In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title_full_unstemmed | In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title_short | In Vitro Assessment of Nanosilver-Functionalized PMMA Bone Cement on Primary Human Mesenchymal Stem Cells and Osteoblasts |
title_sort | in vitro assessment of nanosilver-functionalized pmma bone cement on primary human mesenchymal stem cells and osteoblasts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259364/ https://www.ncbi.nlm.nih.gov/pubmed/25485700 http://dx.doi.org/10.1371/journal.pone.0114740 |
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