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Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity

Forkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family. High expression of FoxQ1 has been associated with several cancers including non-small cell lung cancer (NSCLC), but its role in the development of NSCLC is not clear. In this study, we investigated the effect of FoxQ1 u...

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Autores principales: Feng, Jian, Xu, Liqin, Ni, Songshi, Gu, Jun, Zhu, Huijun, Wang, Haiying, Zhang, Shu, Zhang, Wei, Huang, Jianfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259430/
https://www.ncbi.nlm.nih.gov/pubmed/25356753
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author Feng, Jian
Xu, Liqin
Ni, Songshi
Gu, Jun
Zhu, Huijun
Wang, Haiying
Zhang, Shu
Zhang, Wei
Huang, Jianfei
author_facet Feng, Jian
Xu, Liqin
Ni, Songshi
Gu, Jun
Zhu, Huijun
Wang, Haiying
Zhang, Shu
Zhang, Wei
Huang, Jianfei
author_sort Feng, Jian
collection PubMed
description Forkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family. High expression of FoxQ1 has been associated with several cancers including non-small cell lung cancer (NSCLC), but its role in the development of NSCLC is not clear. In this study, we investigated the effect of FoxQ1 up-regulated and down-regulated in vitro and in vivo, and the role of FoxQ1 in regulating epithelial-mesenchymal transition (EMT) in NSCLC, providing evidence that FoxQ1 could be a potential therapeutic target in NSCLC. NSCLC cells with silenced FoxQ1 had decreased cell proliferation, migration and invasion in cell culture and delayed growth of xenograft tumors in mice compared with corresponding control cells. The NSCLC cells downregulated for FoxQ1 induced the expression of apoptosis-associated proteins and reduction of anti-apoptotic protein expression. Downregulation of FoxQ1 promoted the expression of epithelial markers and decreased several mesenchymal markers in vitro and in vivo. In addition, FoxQ1 was associated with resistance to conventional chemotherapeutic agents. In contrast, FoxQ1 overexpressed elicited converse effects on these phenotypes in vitro and in vivo. Our findings define a key role for FoxQ1 in regulating EMT and increasing chemosensitivity in NSCLC.
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spelling pubmed-42594302014-12-10 Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity Feng, Jian Xu, Liqin Ni, Songshi Gu, Jun Zhu, Huijun Wang, Haiying Zhang, Shu Zhang, Wei Huang, Jianfei Oncotarget Research Paper Forkhead box Q1 (FoxQ1) is a member of the forkhead transcription factor family. High expression of FoxQ1 has been associated with several cancers including non-small cell lung cancer (NSCLC), but its role in the development of NSCLC is not clear. In this study, we investigated the effect of FoxQ1 up-regulated and down-regulated in vitro and in vivo, and the role of FoxQ1 in regulating epithelial-mesenchymal transition (EMT) in NSCLC, providing evidence that FoxQ1 could be a potential therapeutic target in NSCLC. NSCLC cells with silenced FoxQ1 had decreased cell proliferation, migration and invasion in cell culture and delayed growth of xenograft tumors in mice compared with corresponding control cells. The NSCLC cells downregulated for FoxQ1 induced the expression of apoptosis-associated proteins and reduction of anti-apoptotic protein expression. Downregulation of FoxQ1 promoted the expression of epithelial markers and decreased several mesenchymal markers in vitro and in vivo. In addition, FoxQ1 was associated with resistance to conventional chemotherapeutic agents. In contrast, FoxQ1 overexpressed elicited converse effects on these phenotypes in vitro and in vivo. Our findings define a key role for FoxQ1 in regulating EMT and increasing chemosensitivity in NSCLC. Impact Journals LLC 2014-06-13 /pmc/articles/PMC4259430/ /pubmed/25356753 Text en Copyright: © 2014 Feng et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Feng, Jian
Xu, Liqin
Ni, Songshi
Gu, Jun
Zhu, Huijun
Wang, Haiying
Zhang, Shu
Zhang, Wei
Huang, Jianfei
Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title_full Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title_fullStr Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title_full_unstemmed Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title_short Involvement of FoxQ1 in NSCLC through regulating EMT and increasing chemosensitivity
title_sort involvement of foxq1 in nsclc through regulating emt and increasing chemosensitivity
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259430/
https://www.ncbi.nlm.nih.gov/pubmed/25356753
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