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Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)

• Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast geno...

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Autores principales: von Cräutlein, Maria, Korpelainen, Helena, Helander, Marjo, Väre, Henry, Saikkonen, Kari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259459/
https://www.ncbi.nlm.nih.gov/pubmed/25506524
http://dx.doi.org/10.3732/apps.1400094
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author von Cräutlein, Maria
Korpelainen, Helena
Helander, Marjo
Väre, Henry
Saikkonen, Kari
author_facet von Cräutlein, Maria
Korpelainen, Helena
Helander, Marjo
Väre, Henry
Saikkonen, Kari
author_sort von Cräutlein, Maria
collection PubMed
description • Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses.
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spelling pubmed-42594592014-12-12 Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Väre, Henry Saikkonen, Kari Appl Plant Sci Primer Note • Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses. Botanical Society of America 2014-12-04 /pmc/articles/PMC4259459/ /pubmed/25506524 http://dx.doi.org/10.3732/apps.1400094 Text en © 2014 Cräutlein et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
von Cräutlein, Maria
Korpelainen, Helena
Helander, Marjo
Väre, Henry
Saikkonen, Kari
Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title_full Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title_fullStr Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title_full_unstemmed Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title_short Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
title_sort development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, festuca rubra (poaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259459/
https://www.ncbi.nlm.nih.gov/pubmed/25506524
http://dx.doi.org/10.3732/apps.1400094
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