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Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1)
• Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast geno...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259459/ https://www.ncbi.nlm.nih.gov/pubmed/25506524 http://dx.doi.org/10.3732/apps.1400094 |
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author | von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Väre, Henry Saikkonen, Kari |
author_facet | von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Väre, Henry Saikkonen, Kari |
author_sort | von Cräutlein, Maria |
collection | PubMed |
description | • Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses. |
format | Online Article Text |
id | pubmed-4259459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-42594592014-12-12 Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Väre, Henry Saikkonen, Kari Appl Plant Sci Primer Note • Premise of the study: Chloroplast microsatellite markers were developed for Festuca rubra to examine its population genetic characteristics, taxonomy, and coevolution with its endophyte Epichloë festucae. • Methods and Results: Thirteen polymorphic markers were identified from the chloroplast genome of a F. ovina accession and intergenic chloroplast sequences of F. rubra accessions. They amplified a total of 65 alleles in a sample of 93 individuals of F. rubra originating from six different populations located in the Faroe Islands, Finland, Greenland, Norway, and Spain. • Conclusions: The developed microsatellite primer pairs can be used by researchers in population genetic and taxonomic studies, and by plant breeders in breeding programs on grasses. Botanical Society of America 2014-12-04 /pmc/articles/PMC4259459/ /pubmed/25506524 http://dx.doi.org/10.3732/apps.1400094 Text en © 2014 Cräutlein et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note von Cräutlein, Maria Korpelainen, Helena Helander, Marjo Väre, Henry Saikkonen, Kari Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title | Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title_full | Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title_fullStr | Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title_full_unstemmed | Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title_short | Development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, Festuca rubra (Poaceae)(1) |
title_sort | development and characterization of chloroplast microsatellite markers in a fine-leaved fescue, festuca rubra (poaceae)(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259459/ https://www.ncbi.nlm.nih.gov/pubmed/25506524 http://dx.doi.org/10.3732/apps.1400094 |
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