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Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity

Resident bacterial communities (microbiota) and host antimicrobial peptides (AMPs) are both essential components of normal host innate immune responses that limit infection and pathogen induced inflammation. However, their interdependence has not been investigated in the context of urinary tract inf...

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Autores principales: Nienhouse, Vanessa, Gao, Xiang, Dong, Qunfeng, Nelson, David E., Toh, Evelyn, McKinley, Kathleen, Schreckenberger, Paul, Shibata, Noriko, Fok, Cynthia S., Mueller, Elizabeth R., Brubaker, Linda, Wolfe, Alan J., Radek, Katherine A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259481/
https://www.ncbi.nlm.nih.gov/pubmed/25486068
http://dx.doi.org/10.1371/journal.pone.0114185
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author Nienhouse, Vanessa
Gao, Xiang
Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
McKinley, Kathleen
Schreckenberger, Paul
Shibata, Noriko
Fok, Cynthia S.
Mueller, Elizabeth R.
Brubaker, Linda
Wolfe, Alan J.
Radek, Katherine A.
author_facet Nienhouse, Vanessa
Gao, Xiang
Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
McKinley, Kathleen
Schreckenberger, Paul
Shibata, Noriko
Fok, Cynthia S.
Mueller, Elizabeth R.
Brubaker, Linda
Wolfe, Alan J.
Radek, Katherine A.
author_sort Nienhouse, Vanessa
collection PubMed
description Resident bacterial communities (microbiota) and host antimicrobial peptides (AMPs) are both essential components of normal host innate immune responses that limit infection and pathogen induced inflammation. However, their interdependence has not been investigated in the context of urinary tract infection (UTI) susceptibility. Here, we explored the interrelationship between the urinary microbiota and host AMP responses as mechanisms for UTI risk. Using prospectively collected day of surgery (DOS) urine specimens from female pelvic floor surgery participants, we report that the relative abundance and/or frequency of specific urinary microbiota distinguished between participants who did or did not develop a post-operative UTI. Furthermore, UTI risk significantly correlated with both specific urinary microbiota and β-defensin AMP levels. Finally, urinary AMP hydrophobicity and protease activity were greater in participants who developed UTI, and correlated positively with both UTI risk and pelvic floor symptoms. These data demonstrate an interdependency between the urinary microbiota, AMP responses and symptoms, and identify a potential mechanism for UTI risk. Assessment of bacterial microbiota and host innate immune AMP responses in parallel may identify increased risk of UTI in certain populations.
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spelling pubmed-42594812014-12-15 Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity Nienhouse, Vanessa Gao, Xiang Dong, Qunfeng Nelson, David E. Toh, Evelyn McKinley, Kathleen Schreckenberger, Paul Shibata, Noriko Fok, Cynthia S. Mueller, Elizabeth R. Brubaker, Linda Wolfe, Alan J. Radek, Katherine A. PLoS One Research Article Resident bacterial communities (microbiota) and host antimicrobial peptides (AMPs) are both essential components of normal host innate immune responses that limit infection and pathogen induced inflammation. However, their interdependence has not been investigated in the context of urinary tract infection (UTI) susceptibility. Here, we explored the interrelationship between the urinary microbiota and host AMP responses as mechanisms for UTI risk. Using prospectively collected day of surgery (DOS) urine specimens from female pelvic floor surgery participants, we report that the relative abundance and/or frequency of specific urinary microbiota distinguished between participants who did or did not develop a post-operative UTI. Furthermore, UTI risk significantly correlated with both specific urinary microbiota and β-defensin AMP levels. Finally, urinary AMP hydrophobicity and protease activity were greater in participants who developed UTI, and correlated positively with both UTI risk and pelvic floor symptoms. These data demonstrate an interdependency between the urinary microbiota, AMP responses and symptoms, and identify a potential mechanism for UTI risk. Assessment of bacterial microbiota and host innate immune AMP responses in parallel may identify increased risk of UTI in certain populations. Public Library of Science 2014-12-08 /pmc/articles/PMC4259481/ /pubmed/25486068 http://dx.doi.org/10.1371/journal.pone.0114185 Text en © 2014 Nienhouse et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nienhouse, Vanessa
Gao, Xiang
Dong, Qunfeng
Nelson, David E.
Toh, Evelyn
McKinley, Kathleen
Schreckenberger, Paul
Shibata, Noriko
Fok, Cynthia S.
Mueller, Elizabeth R.
Brubaker, Linda
Wolfe, Alan J.
Radek, Katherine A.
Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title_full Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title_fullStr Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title_full_unstemmed Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title_short Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity
title_sort interplay between bladder microbiota and urinary antimicrobial peptides: mechanisms for human urinary tract infection risk and symptom severity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259481/
https://www.ncbi.nlm.nih.gov/pubmed/25486068
http://dx.doi.org/10.1371/journal.pone.0114185
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