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Smooth Muscle Strips for Intestinal Tissue Engineering

Functionally contracting smooth muscle is an essential part of the engineered intestine that has not been replicated in vitro. The purpose of this study is to produce contracting smooth muscle in culture by maintaining the native smooth muscle organization. We employed intact smooth muscle strips an...

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Detalles Bibliográficos
Autores principales: Walthers, Christopher M., Lee, Min, Wu, Benjamin M., Dunn, James C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259486/
https://www.ncbi.nlm.nih.gov/pubmed/25486279
http://dx.doi.org/10.1371/journal.pone.0114850
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author Walthers, Christopher M.
Lee, Min
Wu, Benjamin M.
Dunn, James C. Y.
author_facet Walthers, Christopher M.
Lee, Min
Wu, Benjamin M.
Dunn, James C. Y.
author_sort Walthers, Christopher M.
collection PubMed
description Functionally contracting smooth muscle is an essential part of the engineered intestine that has not been replicated in vitro. The purpose of this study is to produce contracting smooth muscle in culture by maintaining the native smooth muscle organization. We employed intact smooth muscle strips and compared them to dissociated smooth muscle cells in culture for 14 days. Cells isolated by enzymatic digestion quickly lost maturity markers for smooth muscle cells and contained few enteric neural and glial cells. Cultured smooth muscle strips exhibited periodic contraction and maintained neural and glial markers. Smooth muscle strips cultured for 14 days also exhibited regular fluctuation of intracellular calcium, whereas cultured smooth muscle cells did not. After implantation in omentum for 14 days on polycaprolactone scaffolds, smooth muscle strip constructs expressed high levels of smooth muscle maturity markers as well as enteric neural and glial cells. Intact smooth muscle strips may be a useful component for engineered intestinal smooth muscle.
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spelling pubmed-42594862014-12-15 Smooth Muscle Strips for Intestinal Tissue Engineering Walthers, Christopher M. Lee, Min Wu, Benjamin M. Dunn, James C. Y. PLoS One Research Article Functionally contracting smooth muscle is an essential part of the engineered intestine that has not been replicated in vitro. The purpose of this study is to produce contracting smooth muscle in culture by maintaining the native smooth muscle organization. We employed intact smooth muscle strips and compared them to dissociated smooth muscle cells in culture for 14 days. Cells isolated by enzymatic digestion quickly lost maturity markers for smooth muscle cells and contained few enteric neural and glial cells. Cultured smooth muscle strips exhibited periodic contraction and maintained neural and glial markers. Smooth muscle strips cultured for 14 days also exhibited regular fluctuation of intracellular calcium, whereas cultured smooth muscle cells did not. After implantation in omentum for 14 days on polycaprolactone scaffolds, smooth muscle strip constructs expressed high levels of smooth muscle maturity markers as well as enteric neural and glial cells. Intact smooth muscle strips may be a useful component for engineered intestinal smooth muscle. Public Library of Science 2014-12-08 /pmc/articles/PMC4259486/ /pubmed/25486279 http://dx.doi.org/10.1371/journal.pone.0114850 Text en © 2014 Walthers et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Walthers, Christopher M.
Lee, Min
Wu, Benjamin M.
Dunn, James C. Y.
Smooth Muscle Strips for Intestinal Tissue Engineering
title Smooth Muscle Strips for Intestinal Tissue Engineering
title_full Smooth Muscle Strips for Intestinal Tissue Engineering
title_fullStr Smooth Muscle Strips for Intestinal Tissue Engineering
title_full_unstemmed Smooth Muscle Strips for Intestinal Tissue Engineering
title_short Smooth Muscle Strips for Intestinal Tissue Engineering
title_sort smooth muscle strips for intestinal tissue engineering
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259486/
https://www.ncbi.nlm.nih.gov/pubmed/25486279
http://dx.doi.org/10.1371/journal.pone.0114850
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