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Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats

BACKGROUND: The effects of moclobemide on damaged ovarian tissue induced by ischemia- reperfusion and damaged contralateral ovarian tissue were investigated in rats, biochemically and histologically. MATERIALS AND METHODS: In this experimental study, 40 rats were equally divided into four groups: 10...

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Autores principales: Ingec, Metin, Calik, Muhammet, Gundogdu, Cemal, Kurt, Ali, Yilmaz, Mehmet, Isaoglu, Unal, Salman, Suleyman, Akcay, Fatih, Suleyman, Halis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260643/
https://www.ncbi.nlm.nih.gov/pubmed/25505507
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author Ingec, Metin
Calik, Muhammet
Gundogdu, Cemal
Kurt, Ali
Yilmaz, Mehmet
Isaoglu, Unal
Salman, Suleyman
Akcay, Fatih
Suleyman, Halis
author_facet Ingec, Metin
Calik, Muhammet
Gundogdu, Cemal
Kurt, Ali
Yilmaz, Mehmet
Isaoglu, Unal
Salman, Suleyman
Akcay, Fatih
Suleyman, Halis
author_sort Ingec, Metin
collection PubMed
description BACKGROUND: The effects of moclobemide on damaged ovarian tissue induced by ischemia- reperfusion and damaged contralateral ovarian tissue were investigated in rats, biochemically and histologically. MATERIALS AND METHODS: In this experimental study, 40 rats were equally divided into four groups: 10 mg/kg moclobemide, 20 mg/kg moclobemide, ischemia/reperfusion control, and intact control groups. A 2-2.5-cm-long vertical incision was made in the lower abdomen of each rat in order to reach the ovaries, after which a vascular clip was placed on the lower side of the right ovary of each animal in the two treatment groups and the ischemia-reperfusion control group, but not in the healthy (intact control) animal group. The purpose of this procedure was to create ischemia over the course of three hours, then the clips were unclamped to provide reperfusion for the next two hours. At the end of the two hours of reperfusion, all the animals were killed by high-dose anaesthesia and their ovaries were taken and subjected to histological and biochemical (malondialdehyde, nitric oxide, glutathione) studies. RESULTS: The obtained results showed that moclobemide suppressed nitric oxide and malondialdehyde production in the ischemia-reperfusion damage area, and prevented the decrease in endogenous antioxidant levels (glutathione) in the rat ovarian tissue. Moclobemide also prevented infiltration of leukocytes to the ovarian tissue. These results showed that moclobemide protected ovarian tissue against ischemiareperfusion injury. CONCLUSION: This study shows that moclobemide represses malondialdehyde and nitric oxide production in the rat ovarian tissue subjected to ischemia-reperfusion injury and keeps the endogenous antioxidant glutathione level from decreasing. Moclobemide also inhibits leukocytic migration into ovarian tissue following ischemia-reperfusion injury. From these results, it is suggested that moclobemide can be used in the treatment of ovarian ischemia-reperfusion injury.
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spelling pubmed-42606432014-12-10 Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats Ingec, Metin Calik, Muhammet Gundogdu, Cemal Kurt, Ali Yilmaz, Mehmet Isaoglu, Unal Salman, Suleyman Akcay, Fatih Suleyman, Halis Int J Fertil Steril Research Article BACKGROUND: The effects of moclobemide on damaged ovarian tissue induced by ischemia- reperfusion and damaged contralateral ovarian tissue were investigated in rats, biochemically and histologically. MATERIALS AND METHODS: In this experimental study, 40 rats were equally divided into four groups: 10 mg/kg moclobemide, 20 mg/kg moclobemide, ischemia/reperfusion control, and intact control groups. A 2-2.5-cm-long vertical incision was made in the lower abdomen of each rat in order to reach the ovaries, after which a vascular clip was placed on the lower side of the right ovary of each animal in the two treatment groups and the ischemia-reperfusion control group, but not in the healthy (intact control) animal group. The purpose of this procedure was to create ischemia over the course of three hours, then the clips were unclamped to provide reperfusion for the next two hours. At the end of the two hours of reperfusion, all the animals were killed by high-dose anaesthesia and their ovaries were taken and subjected to histological and biochemical (malondialdehyde, nitric oxide, glutathione) studies. RESULTS: The obtained results showed that moclobemide suppressed nitric oxide and malondialdehyde production in the ischemia-reperfusion damage area, and prevented the decrease in endogenous antioxidant levels (glutathione) in the rat ovarian tissue. Moclobemide also prevented infiltration of leukocytes to the ovarian tissue. These results showed that moclobemide protected ovarian tissue against ischemiareperfusion injury. CONCLUSION: This study shows that moclobemide represses malondialdehyde and nitric oxide production in the rat ovarian tissue subjected to ischemia-reperfusion injury and keeps the endogenous antioxidant glutathione level from decreasing. Moclobemide also inhibits leukocytic migration into ovarian tissue following ischemia-reperfusion injury. From these results, it is suggested that moclobemide can be used in the treatment of ovarian ischemia-reperfusion injury. Royan Institute 2012 2012-06-19 /pmc/articles/PMC4260643/ /pubmed/25505507 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ingec, Metin
Calik, Muhammet
Gundogdu, Cemal
Kurt, Ali
Yilmaz, Mehmet
Isaoglu, Unal
Salman, Suleyman
Akcay, Fatih
Suleyman, Halis
Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title_full Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title_fullStr Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title_full_unstemmed Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title_short Biological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
title_sort biological and histopathological investigations of moclobemide on injured ovarian tissue following induction of ischemia-reperfusion in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260643/
https://www.ncbi.nlm.nih.gov/pubmed/25505507
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