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The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control
RNA interference (RNAi) has great potential for use in insect pest control. However, some significant challenges must be overcome before RNAi-based pest control can become a reality. One challenge is the proper selection of a good target gene for RNAi. Here, we report that the insect ecdysone recept...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261201/ https://www.ncbi.nlm.nih.gov/pubmed/25516715 http://dx.doi.org/10.7150/ijbs.9598 |
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author | Yu, Rong Xu, Xinping Liang, Yongkang Tian, Honggang Pan, Zhanqing Jin, Shouheng Wang, Na Zhang, Wenqing |
author_facet | Yu, Rong Xu, Xinping Liang, Yongkang Tian, Honggang Pan, Zhanqing Jin, Shouheng Wang, Na Zhang, Wenqing |
author_sort | Yu, Rong |
collection | PubMed |
description | RNA interference (RNAi) has great potential for use in insect pest control. However, some significant challenges must be overcome before RNAi-based pest control can become a reality. One challenge is the proper selection of a good target gene for RNAi. Here, we report that the insect ecdysone receptor (EcR) is a good potential target for RNAi-based pest control in the brown planthopper Nilaparvata lugens, a serious insect pest of rice plants. We demonstrated that the use of a 360 bp fragment (NlEcR-c) that is common between NlEcR-A and NlEcR-B for feeding RNAi experiments significantly decreased the relative mRNA expression levels of NlEcR compared with those in the dsGFP control. Feeding RNAi also resulted in a significant reduction in the number of offspring per pair of N. lugens. Consequently, a transgenic rice line expressing NlEcR dsRNA was constructed by Agrobacterium- mediated transformation. The results of qRT-PCR showed that the total copy number of the target gene in all transgenic rice lines was 2. Northern blot analysis showed that the small RNA of the hairpin dsNlEcR-c was successfully expressed in the transgenic rice lines. After newly hatched nymphs of N. lugens fed on the transgenic rice lines, effective RNAi was observed. The NlEcR expression levels in all lines examined were decreased significantly compared with the control. In all lines, the survival rate of the nymphs was nearly 90%, and the average number of offspring per pair in the treated groups was significantly less than that observed in the control, with a decrease of 44.18-66.27%. These findings support an RNAi-based pest control strategy and are also important for the management of rice insect pests. |
format | Online Article Text |
id | pubmed-4261201 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-42612012014-12-16 The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control Yu, Rong Xu, Xinping Liang, Yongkang Tian, Honggang Pan, Zhanqing Jin, Shouheng Wang, Na Zhang, Wenqing Int J Biol Sci Research Paper RNA interference (RNAi) has great potential for use in insect pest control. However, some significant challenges must be overcome before RNAi-based pest control can become a reality. One challenge is the proper selection of a good target gene for RNAi. Here, we report that the insect ecdysone receptor (EcR) is a good potential target for RNAi-based pest control in the brown planthopper Nilaparvata lugens, a serious insect pest of rice plants. We demonstrated that the use of a 360 bp fragment (NlEcR-c) that is common between NlEcR-A and NlEcR-B for feeding RNAi experiments significantly decreased the relative mRNA expression levels of NlEcR compared with those in the dsGFP control. Feeding RNAi also resulted in a significant reduction in the number of offspring per pair of N. lugens. Consequently, a transgenic rice line expressing NlEcR dsRNA was constructed by Agrobacterium- mediated transformation. The results of qRT-PCR showed that the total copy number of the target gene in all transgenic rice lines was 2. Northern blot analysis showed that the small RNA of the hairpin dsNlEcR-c was successfully expressed in the transgenic rice lines. After newly hatched nymphs of N. lugens fed on the transgenic rice lines, effective RNAi was observed. The NlEcR expression levels in all lines examined were decreased significantly compared with the control. In all lines, the survival rate of the nymphs was nearly 90%, and the average number of offspring per pair in the treated groups was significantly less than that observed in the control, with a decrease of 44.18-66.27%. These findings support an RNAi-based pest control strategy and are also important for the management of rice insect pests. Ivyspring International Publisher 2014-11-07 /pmc/articles/PMC4261201/ /pubmed/25516715 http://dx.doi.org/10.7150/ijbs.9598 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Yu, Rong Xu, Xinping Liang, Yongkang Tian, Honggang Pan, Zhanqing Jin, Shouheng Wang, Na Zhang, Wenqing The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title | The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title_full | The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title_fullStr | The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title_full_unstemmed | The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title_short | The Insect Ecdysone Receptor is a Good Potential Target for RNAi-based Pest Control |
title_sort | insect ecdysone receptor is a good potential target for rnai-based pest control |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261201/ https://www.ncbi.nlm.nih.gov/pubmed/25516715 http://dx.doi.org/10.7150/ijbs.9598 |
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