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An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses
The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261831/ https://www.ncbi.nlm.nih.gov/pubmed/25540652 http://dx.doi.org/10.3389/fgene.2014.00437 |
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author | Costa, Pedro M. Pinto, Miguel Vicente, Ana M. Gonçalves, Cátia Rodrigo, Ana P. Louro, Henriqueta Costa, Maria H. Caeiro, Sandra Silva, Maria J. |
author_facet | Costa, Pedro M. Pinto, Miguel Vicente, Ana M. Gonçalves, Cátia Rodrigo, Ana P. Louro, Henriqueta Costa, Maria H. Caeiro, Sandra Silva, Maria J. |
author_sort | Costa, Pedro M. |
collection | PubMed |
description | The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments. |
format | Online Article Text |
id | pubmed-4261831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42618312014-12-24 An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses Costa, Pedro M. Pinto, Miguel Vicente, Ana M. Gonçalves, Cátia Rodrigo, Ana P. Louro, Henriqueta Costa, Maria H. Caeiro, Sandra Silva, Maria J. Front Genet Genetics The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments. Frontiers Media S.A. 2014-12-10 /pmc/articles/PMC4261831/ /pubmed/25540652 http://dx.doi.org/10.3389/fgene.2014.00437 Text en Copyright © 2014 Costa, Pinto, Vicente, Gonçalves, Rodrigo, Louro, Costa, Caeiro and Silva. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Costa, Pedro M. Pinto, Miguel Vicente, Ana M. Gonçalves, Cátia Rodrigo, Ana P. Louro, Henriqueta Costa, Maria H. Caeiro, Sandra Silva, Maria J. An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title | An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title_full | An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title_fullStr | An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title_full_unstemmed | An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title_short | An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
title_sort | integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261831/ https://www.ncbi.nlm.nih.gov/pubmed/25540652 http://dx.doi.org/10.3389/fgene.2014.00437 |
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