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Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper
BACKGROUND: Gastrointestinal nematodes cause significant economic losses in the sheep industry, with frequent reports of anthelmintic resistance. Therefore, alternative methods to control these parasites are necessary. Thus, the aim of the present study was to assess the effect of treatment with sel...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261986/ https://www.ncbi.nlm.nih.gov/pubmed/25096962 http://dx.doi.org/10.1186/1756-3305-7-355 |
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author | Fausto, Guilherme Costa Pivoto, Felipe Lamberti Costa, Márcio Machado dos Anjos Lopes, Sônia Terezinha França, Raqueli Teresinha Molento, Marcelo Beltrão Minervino, Antonio Humberto Hamad da Rocha, João Batista Teixeira do Rêgo Leal, Marta Lizandra |
author_facet | Fausto, Guilherme Costa Pivoto, Felipe Lamberti Costa, Márcio Machado dos Anjos Lopes, Sônia Terezinha França, Raqueli Teresinha Molento, Marcelo Beltrão Minervino, Antonio Humberto Hamad da Rocha, João Batista Teixeira do Rêgo Leal, Marta Lizandra |
author_sort | Fausto, Guilherme Costa |
collection | PubMed |
description | BACKGROUND: Gastrointestinal nematodes cause significant economic losses in the sheep industry, with frequent reports of anthelmintic resistance. Therefore, alternative methods to control these parasites are necessary. Thus, the aim of the present study was to assess the effect of treatment with selenium and copper on the protein profile of sheep that were experimentally infected with Haemonchus contortus. METHODS: Twenty-eight lambs were experimentally infected with H. contortus and divided into four experimental groups as follow: G1 - untreated animals; G2 - treated with sodium selenite; G3 - treated with copper; G4 - treated with sodium selenite and copper. The serum protein, body weight and egg count per gram of feces (EPG) were assessed at the baseline and after 20, 40, 60 and 80 days. The parasite burden was assessed 80 days after the beginning of the experiment. RESULTS: Higher levels of total protein and gamma globulin were observed in the lambs treated with sodium selenite and copper on D80. Copper acted as a growth promoter. The copper-supplemented groups exhibited higher daily and total weight gain. The association of selenium and copper altered the protein profile of sheep. Copper and selenium supplementation reduced EPG and worm burden at the end of the experiment. To the best of our knowledge, this is the first study to demonstrate the positive effect of the combined parenteral supplementation of Se and Cu on H. contortus infection. CONCLUSIONS: This injectable supplementation could be used as an auxiliary method to control H. contortus in sheep. |
format | Online Article Text |
id | pubmed-4261986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42619862014-12-10 Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper Fausto, Guilherme Costa Pivoto, Felipe Lamberti Costa, Márcio Machado dos Anjos Lopes, Sônia Terezinha França, Raqueli Teresinha Molento, Marcelo Beltrão Minervino, Antonio Humberto Hamad da Rocha, João Batista Teixeira do Rêgo Leal, Marta Lizandra Parasit Vectors Research BACKGROUND: Gastrointestinal nematodes cause significant economic losses in the sheep industry, with frequent reports of anthelmintic resistance. Therefore, alternative methods to control these parasites are necessary. Thus, the aim of the present study was to assess the effect of treatment with selenium and copper on the protein profile of sheep that were experimentally infected with Haemonchus contortus. METHODS: Twenty-eight lambs were experimentally infected with H. contortus and divided into four experimental groups as follow: G1 - untreated animals; G2 - treated with sodium selenite; G3 - treated with copper; G4 - treated with sodium selenite and copper. The serum protein, body weight and egg count per gram of feces (EPG) were assessed at the baseline and after 20, 40, 60 and 80 days. The parasite burden was assessed 80 days after the beginning of the experiment. RESULTS: Higher levels of total protein and gamma globulin were observed in the lambs treated with sodium selenite and copper on D80. Copper acted as a growth promoter. The copper-supplemented groups exhibited higher daily and total weight gain. The association of selenium and copper altered the protein profile of sheep. Copper and selenium supplementation reduced EPG and worm burden at the end of the experiment. To the best of our knowledge, this is the first study to demonstrate the positive effect of the combined parenteral supplementation of Se and Cu on H. contortus infection. CONCLUSIONS: This injectable supplementation could be used as an auxiliary method to control H. contortus in sheep. BioMed Central 2014-08-05 /pmc/articles/PMC4261986/ /pubmed/25096962 http://dx.doi.org/10.1186/1756-3305-7-355 Text en © Fausto et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Fausto, Guilherme Costa Pivoto, Felipe Lamberti Costa, Márcio Machado dos Anjos Lopes, Sônia Terezinha França, Raqueli Teresinha Molento, Marcelo Beltrão Minervino, Antonio Humberto Hamad da Rocha, João Batista Teixeira do Rêgo Leal, Marta Lizandra Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title | Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title_full | Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title_fullStr | Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title_full_unstemmed | Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title_short | Protein profile of lambs experimentally infected with Haemonchus contortus and supplemented with selenium and copper |
title_sort | protein profile of lambs experimentally infected with haemonchus contortus and supplemented with selenium and copper |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261986/ https://www.ncbi.nlm.nih.gov/pubmed/25096962 http://dx.doi.org/10.1186/1756-3305-7-355 |
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