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The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite
BACKGROUND: Parascaris univalens is an ascaridoid nematode of equids. Little is known about its epidemiology and population genetics in domestic and wild horse populations. PCR-based methods are suited to support studies in these areas, provided that reliable genetic markers are used. Recent studies...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262126/ https://www.ncbi.nlm.nih.gov/pubmed/25190631 http://dx.doi.org/10.1186/1756-3305-7-428 |
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author | Jabbar, Abdul Littlewood, D Timothy J Mohandas, Namitha Briscoe, Andrew G Foster, Peter G Müller, Fritz von Samson-Himmelstjerna, Georg Jex, Aaron R Gasser, Robin B |
author_facet | Jabbar, Abdul Littlewood, D Timothy J Mohandas, Namitha Briscoe, Andrew G Foster, Peter G Müller, Fritz von Samson-Himmelstjerna, Georg Jex, Aaron R Gasser, Robin B |
author_sort | Jabbar, Abdul |
collection | PubMed |
description | BACKGROUND: Parascaris univalens is an ascaridoid nematode of equids. Little is known about its epidemiology and population genetics in domestic and wild horse populations. PCR-based methods are suited to support studies in these areas, provided that reliable genetic markers are used. Recent studies have shown that mitochondrial (mt) genomic markers are applicable in such methods, but no such markers have been defined for P. univalens. METHODS: Mt genome regions were amplified from total genomic DNA isolated from P. univalens eggs by long-PCR and sequenced using Illumina technology. The mt genome was assembled and annotated using an established bioinformatic pipeline. Amino acid sequences inferred from all protein-encoding genes of the mt genomes were compared with those from other ascaridoid nematodes, and concatenated sequences were subjected to phylogenetic analysis by Bayesian inference. RESULTS: The circular mt genome was 13,920 bp in length and contained two ribosomal RNA, 12 protein-coding and 22 transfer RNA genes, consistent with those of other ascaridoids. Phylogenetic analysis of the concatenated amino acid sequence data for the 12 mt proteins showed that P. univalens was most closely related to Ascaris lumbricoides and A. suum, to the exclusion of other ascaridoids. CONCLUSIONS: This mt genome representing P. univalens now provides a rich source of genetic markers for future studies of the genetics and epidemiology of this parasite and its congener, P. equorum. This focus is significant, given that there is no published information on the specific prevalence and distribution of P. univalens infection in domestic and wild horse populations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-3305-7-428) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4262126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42621262014-12-11 The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite Jabbar, Abdul Littlewood, D Timothy J Mohandas, Namitha Briscoe, Andrew G Foster, Peter G Müller, Fritz von Samson-Himmelstjerna, Georg Jex, Aaron R Gasser, Robin B Parasit Vectors Research BACKGROUND: Parascaris univalens is an ascaridoid nematode of equids. Little is known about its epidemiology and population genetics in domestic and wild horse populations. PCR-based methods are suited to support studies in these areas, provided that reliable genetic markers are used. Recent studies have shown that mitochondrial (mt) genomic markers are applicable in such methods, but no such markers have been defined for P. univalens. METHODS: Mt genome regions were amplified from total genomic DNA isolated from P. univalens eggs by long-PCR and sequenced using Illumina technology. The mt genome was assembled and annotated using an established bioinformatic pipeline. Amino acid sequences inferred from all protein-encoding genes of the mt genomes were compared with those from other ascaridoid nematodes, and concatenated sequences were subjected to phylogenetic analysis by Bayesian inference. RESULTS: The circular mt genome was 13,920 bp in length and contained two ribosomal RNA, 12 protein-coding and 22 transfer RNA genes, consistent with those of other ascaridoids. Phylogenetic analysis of the concatenated amino acid sequence data for the 12 mt proteins showed that P. univalens was most closely related to Ascaris lumbricoides and A. suum, to the exclusion of other ascaridoids. CONCLUSIONS: This mt genome representing P. univalens now provides a rich source of genetic markers for future studies of the genetics and epidemiology of this parasite and its congener, P. equorum. This focus is significant, given that there is no published information on the specific prevalence and distribution of P. univalens infection in domestic and wild horse populations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-3305-7-428) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-04 /pmc/articles/PMC4262126/ /pubmed/25190631 http://dx.doi.org/10.1186/1756-3305-7-428 Text en © Jabbar et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Jabbar, Abdul Littlewood, D Timothy J Mohandas, Namitha Briscoe, Andrew G Foster, Peter G Müller, Fritz von Samson-Himmelstjerna, Georg Jex, Aaron R Gasser, Robin B The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title | The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title_full | The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title_fullStr | The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title_full_unstemmed | The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title_short | The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite |
title_sort | mitochondrial genome of parascaris univalens - implications for a “forgotten” parasite |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262126/ https://www.ncbi.nlm.nih.gov/pubmed/25190631 http://dx.doi.org/10.1186/1756-3305-7-428 |
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