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Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)

BACKGROUND: Although spargana, which are the plerocercoids of Spirometra erinacei, are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, sparganum ESTs were examined by la...

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Autores principales: Kim, Dae-Won, Yoo, Won Gi, Lee, Myoung-Ro, Yang, Hye-Won, Kim, Yu-Jung, Cho, Shin-Hyeong, Lee, Won-Ja, Ju, Jung-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262225/
https://www.ncbi.nlm.nih.gov/pubmed/25128015
http://dx.doi.org/10.1186/1756-3305-7-368
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author Kim, Dae-Won
Yoo, Won Gi
Lee, Myoung-Ro
Yang, Hye-Won
Kim, Yu-Jung
Cho, Shin-Hyeong
Lee, Won-Ja
Ju, Jung-Won
author_facet Kim, Dae-Won
Yoo, Won Gi
Lee, Myoung-Ro
Yang, Hye-Won
Kim, Yu-Jung
Cho, Shin-Hyeong
Lee, Won-Ja
Ju, Jung-Won
author_sort Kim, Dae-Won
collection PubMed
description BACKGROUND: Although spargana, which are the plerocercoids of Spirometra erinacei, are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, sparganum ESTs were examined by large-scale EST sequencing and multiple bioinformatics tools. METHODS: Total RNA was isolated from spargana and then ESTs were generated, assembled and sequenced. Many biological aspects of spargana were investigated using multi-step bioinformatics tools. RESULTS: A total of 5,634 ESTs were collected from spargana. After clustering and assembly, the functions of 1,794 Sparganum Assembled ESTs (SpAEs) including 934 contigs and 860 singletons were analyzed. A total of 1,351 (75%) SpAEs were annotated using a hybrid of BLASTX and InterProScan. Of these genes, 1,041 (58%) SpAEs had high similarity to tapeworms. In the context of the biology of sparganum, our analyses reveal: (i) a highly expressed fibronectin 1, a ubiquitous and abundant glycoprotein; (ii) up-regulation of enzymes related with glycolysis pathway; (iii) most frequent domains of protein kinase and RNA recognition motif domain; (iv) a set of helminth-parasitic and spargana-specific genes that may offer a number of antigen candidates. CONCLUSIONS: Our transcriptomic analysis of S. erinacei spargana demonstrates biological aspects of a parasite that invades and travels through subcutaneous tissue in intermediate hosts. Future studies should include comparative analyses using combinations of transcriptome and proteome data collected from the entire life cycle of S. erinacei. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-3305-7-368) contains supplementary material, which is available to authorized users.
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spelling pubmed-42622252014-12-11 Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids) Kim, Dae-Won Yoo, Won Gi Lee, Myoung-Ro Yang, Hye-Won Kim, Yu-Jung Cho, Shin-Hyeong Lee, Won-Ja Ju, Jung-Won Parasit Vectors Research BACKGROUND: Although spargana, which are the plerocercoids of Spirometra erinacei, are of biological and clinical importance, expressed sequence tags (ESTs) from this parasite have not been explored. To understand molecular and biological features of this parasite, sparganum ESTs were examined by large-scale EST sequencing and multiple bioinformatics tools. METHODS: Total RNA was isolated from spargana and then ESTs were generated, assembled and sequenced. Many biological aspects of spargana were investigated using multi-step bioinformatics tools. RESULTS: A total of 5,634 ESTs were collected from spargana. After clustering and assembly, the functions of 1,794 Sparganum Assembled ESTs (SpAEs) including 934 contigs and 860 singletons were analyzed. A total of 1,351 (75%) SpAEs were annotated using a hybrid of BLASTX and InterProScan. Of these genes, 1,041 (58%) SpAEs had high similarity to tapeworms. In the context of the biology of sparganum, our analyses reveal: (i) a highly expressed fibronectin 1, a ubiquitous and abundant glycoprotein; (ii) up-regulation of enzymes related with glycolysis pathway; (iii) most frequent domains of protein kinase and RNA recognition motif domain; (iv) a set of helminth-parasitic and spargana-specific genes that may offer a number of antigen candidates. CONCLUSIONS: Our transcriptomic analysis of S. erinacei spargana demonstrates biological aspects of a parasite that invades and travels through subcutaneous tissue in intermediate hosts. Future studies should include comparative analyses using combinations of transcriptome and proteome data collected from the entire life cycle of S. erinacei. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1756-3305-7-368) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-15 /pmc/articles/PMC4262225/ /pubmed/25128015 http://dx.doi.org/10.1186/1756-3305-7-368 Text en © Kim et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kim, Dae-Won
Yoo, Won Gi
Lee, Myoung-Ro
Yang, Hye-Won
Kim, Yu-Jung
Cho, Shin-Hyeong
Lee, Won-Ja
Ju, Jung-Won
Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title_full Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title_fullStr Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title_full_unstemmed Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title_short Transcriptome sequencing and analysis of the zoonotic parasite Spirometra erinacei spargana (plerocercoids)
title_sort transcriptome sequencing and analysis of the zoonotic parasite spirometra erinacei spargana (plerocercoids)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262225/
https://www.ncbi.nlm.nih.gov/pubmed/25128015
http://dx.doi.org/10.1186/1756-3305-7-368
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