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Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection
Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray t...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262287/ https://www.ncbi.nlm.nih.gov/pubmed/25506299 http://dx.doi.org/10.5423/PPJ.OA.06.2014.0055 |
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author | Wang, Yiming Kwon, Soon Jae Wu, Jingni Choi, Jaeyoung Lee, Yong-Hwan Agrawal, Ganesh Kumar Tamogami, Shigeru Rakwal, Randeep Park, Sang-Ryeol Kim, Beom-Gi Jung, Ki-Hong Kang, Kyu Young Kim, Sang Gon Kim, Sun Tae |
author_facet | Wang, Yiming Kwon, Soon Jae Wu, Jingni Choi, Jaeyoung Lee, Yong-Hwan Agrawal, Ganesh Kumar Tamogami, Shigeru Rakwal, Randeep Park, Sang-Ryeol Kim, Beom-Gi Jung, Ki-Hong Kang, Kyu Young Kim, Sang Gon Kim, Sun Tae |
author_sort | Wang, Yiming |
collection | PubMed |
description | Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack. |
format | Online Article Text |
id | pubmed-4262287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42622872014-12-12 Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection Wang, Yiming Kwon, Soon Jae Wu, Jingni Choi, Jaeyoung Lee, Yong-Hwan Agrawal, Ganesh Kumar Tamogami, Shigeru Rakwal, Randeep Park, Sang-Ryeol Kim, Beom-Gi Jung, Ki-Hong Kang, Kyu Young Kim, Sang Gon Kim, Sun Tae Plant Pathol J Articles Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack. Korean Society of Plant Pathology 2014-12 2014-12-15 /pmc/articles/PMC4262287/ /pubmed/25506299 http://dx.doi.org/10.5423/PPJ.OA.06.2014.0055 Text en © The Korean Society of Plant Pathology |
spellingShingle | Articles Wang, Yiming Kwon, Soon Jae Wu, Jingni Choi, Jaeyoung Lee, Yong-Hwan Agrawal, Ganesh Kumar Tamogami, Shigeru Rakwal, Randeep Park, Sang-Ryeol Kim, Beom-Gi Jung, Ki-Hong Kang, Kyu Young Kim, Sang Gon Kim, Sun Tae Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title | Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title_full | Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title_fullStr | Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title_full_unstemmed | Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title_short | Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection |
title_sort | transcriptome analysis of early responsive genes in rice during magnaporthe oryzae infection |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262287/ https://www.ncbi.nlm.nih.gov/pubmed/25506299 http://dx.doi.org/10.5423/PPJ.OA.06.2014.0055 |
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