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Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1

Fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their ATP-driven efflux pumps belonging to ATP-binding cassette (ABC) transporters. To clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicide...

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Autores principales: Kim, Suyoung, Park, Sook-Young, Kim, Hyejeong, Kim, Dongyoung, Lee, Seon-Woo, Kim, Heung Tae, Lee, Jong-Hwan, Choi, Woobong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Plant Pathology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262290/
https://www.ncbi.nlm.nih.gov/pubmed/25506302
http://dx.doi.org/10.5423/PPJ.OA.08.2014.0077
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author Kim, Suyoung
Park, Sook-Young
Kim, Hyejeong
Kim, Dongyoung
Lee, Seon-Woo
Kim, Heung Tae
Lee, Jong-Hwan
Choi, Woobong
author_facet Kim, Suyoung
Park, Sook-Young
Kim, Hyejeong
Kim, Dongyoung
Lee, Seon-Woo
Kim, Heung Tae
Lee, Jong-Hwan
Choi, Woobong
author_sort Kim, Suyoung
collection PubMed
description Fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their ATP-driven efflux pumps belonging to ATP-binding cassette (ABC) transporters. To clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicides, we constructed a cDNA library from germinated conidia of Colletotrichum acutatum, a major anthracnose pathogen of pepper (Capsicum annum L.). Over 1,000 cDNA clones were sequenced, of which single clone exhibited significant nucleotide sequence homology to ABC transporter genes. We isolated three fosmid clones containing the C. acutatum ABC1 (CaABC1) gene in full-length from genomic DNA library screening. The CaABC1 gene consists of 4,059 bp transcript, predicting a 1,353-aa protein. The gene contains the typical ABC signature and Walker A and B motifs. The 5′-flanking region contains a CAAT motif, a TATA box, and a Kozak region. Phylogenetic and structural analysis suggested that the CaABC1 is a typical ABC transporter gene highly conserved in various fungal species, as well as in Chromista, Metazoans, and Viridiplantae. We also found that CaABC1 was up-regulated during conidiation and a minimal medium condition. Moreover, CaABC1 was induced in iprobenfos, kresoxim-methyl, thiophanate-methyl, and hygromycin B. These results demonstrate that CaABC1 is necessary for conidiation, abiotic stress, and various fungicide resistances. These results will provide the basis for further study on the function of ABC transporter genes in C. acutatum.
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spelling pubmed-42622902014-12-12 Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1 Kim, Suyoung Park, Sook-Young Kim, Hyejeong Kim, Dongyoung Lee, Seon-Woo Kim, Heung Tae Lee, Jong-Hwan Choi, Woobong Plant Pathol J Articles Fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their ATP-driven efflux pumps belonging to ATP-binding cassette (ABC) transporters. To clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicides, we constructed a cDNA library from germinated conidia of Colletotrichum acutatum, a major anthracnose pathogen of pepper (Capsicum annum L.). Over 1,000 cDNA clones were sequenced, of which single clone exhibited significant nucleotide sequence homology to ABC transporter genes. We isolated three fosmid clones containing the C. acutatum ABC1 (CaABC1) gene in full-length from genomic DNA library screening. The CaABC1 gene consists of 4,059 bp transcript, predicting a 1,353-aa protein. The gene contains the typical ABC signature and Walker A and B motifs. The 5′-flanking region contains a CAAT motif, a TATA box, and a Kozak region. Phylogenetic and structural analysis suggested that the CaABC1 is a typical ABC transporter gene highly conserved in various fungal species, as well as in Chromista, Metazoans, and Viridiplantae. We also found that CaABC1 was up-regulated during conidiation and a minimal medium condition. Moreover, CaABC1 was induced in iprobenfos, kresoxim-methyl, thiophanate-methyl, and hygromycin B. These results demonstrate that CaABC1 is necessary for conidiation, abiotic stress, and various fungicide resistances. These results will provide the basis for further study on the function of ABC transporter genes in C. acutatum. Korean Society of Plant Pathology 2014-12 2014-12-15 /pmc/articles/PMC4262290/ /pubmed/25506302 http://dx.doi.org/10.5423/PPJ.OA.08.2014.0077 Text en © The Korean Society of Plant Pathology
spellingShingle Articles
Kim, Suyoung
Park, Sook-Young
Kim, Hyejeong
Kim, Dongyoung
Lee, Seon-Woo
Kim, Heung Tae
Lee, Jong-Hwan
Choi, Woobong
Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title_full Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title_fullStr Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title_full_unstemmed Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title_short Isolation and Characterization of the Colletotrichum acutatum ABC Transporter CaABC1
title_sort isolation and characterization of the colletotrichum acutatum abc transporter caabc1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262290/
https://www.ncbi.nlm.nih.gov/pubmed/25506302
http://dx.doi.org/10.5423/PPJ.OA.08.2014.0077
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