Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture

We recently reported that the overexpression of GroEL2 played an important role in increasing the alkane tolerance of Rhodococcus erythropolis PR4. In the present study, we examined the effects of the introduction of groEL2 on the alkane tolerance of other Rhodococcus strains. The introduction of gr...

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Autores principales: Takihara, Hayato, Matsuura, Chiaki, Ogihara, Jun, Iwabuchi, Noriyuki, Sunairi, Michio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262369/
https://www.ncbi.nlm.nih.gov/pubmed/25491752
http://dx.doi.org/10.1264/jsme2.ME14114
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author Takihara, Hayato
Matsuura, Chiaki
Ogihara, Jun
Iwabuchi, Noriyuki
Sunairi, Michio
author_facet Takihara, Hayato
Matsuura, Chiaki
Ogihara, Jun
Iwabuchi, Noriyuki
Sunairi, Michio
author_sort Takihara, Hayato
collection PubMed
description We recently reported that the overexpression of GroEL2 played an important role in increasing the alkane tolerance of Rhodococcus erythropolis PR4. In the present study, we examined the effects of the introduction of groEL2 on the alkane tolerance of other Rhodococcus strains. The introduction of groEL2 into Rhodococcus strains led to increased alkane tolerance. The translocation of R. rhodochrous ATCC12674 cells to and survival in the n-octane (C8) phase in two phase culture were significantly enhanced by the introduction of groEL2 derived from strain PR4, suggesting that engineering cells to overexpress GroEL2 represents an effective strategy for enhancing organic solvent tolerance in Rhodococcus.
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spelling pubmed-42623692014-12-16 Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture Takihara, Hayato Matsuura, Chiaki Ogihara, Jun Iwabuchi, Noriyuki Sunairi, Michio Microbes Environ Short Communication We recently reported that the overexpression of GroEL2 played an important role in increasing the alkane tolerance of Rhodococcus erythropolis PR4. In the present study, we examined the effects of the introduction of groEL2 on the alkane tolerance of other Rhodococcus strains. The introduction of groEL2 into Rhodococcus strains led to increased alkane tolerance. The translocation of R. rhodochrous ATCC12674 cells to and survival in the n-octane (C8) phase in two phase culture were significantly enhanced by the introduction of groEL2 derived from strain PR4, suggesting that engineering cells to overexpress GroEL2 represents an effective strategy for enhancing organic solvent tolerance in Rhodococcus. The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM) 2014-12 2014-12-10 /pmc/articles/PMC4262369/ /pubmed/25491752 http://dx.doi.org/10.1264/jsme2.ME14114 Text en Copyright 2014 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Takihara, Hayato
Matsuura, Chiaki
Ogihara, Jun
Iwabuchi, Noriyuki
Sunairi, Michio
Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title_full Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title_fullStr Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title_full_unstemmed Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title_short Rhodococcus rhodochrous ATCC12674 Becomes Alkane-Tolerant upon GroEL2 Overexpression and Survives in the n-Octane Phase in Two Phase Culture
title_sort rhodococcus rhodochrous atcc12674 becomes alkane-tolerant upon groel2 overexpression and survives in the n-octane phase in two phase culture
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262369/
https://www.ncbi.nlm.nih.gov/pubmed/25491752
http://dx.doi.org/10.1264/jsme2.ME14114
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