Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei

Carbon storage is likely to enable adaptation of trypanosomes to nutritional challenges or bottlenecks during their stage development and migration in the tsetse. Lipid droplets are candidates for this function. This report shows that feeding of T. brucei with oleate results in a 4–5 fold increase i...

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Autores principales: Allmann, Stefan, Mazet, Muriel, Ziebart, Nicole, Bouyssou, Guillaume, Fouillen, Laetitia, Dupuy, Jean-William, Bonneu, Marc, Moreau, Patrick, Bringaud, Frédéric, Boshart, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262433/
https://www.ncbi.nlm.nih.gov/pubmed/25493940
http://dx.doi.org/10.1371/journal.pone.0114628
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author Allmann, Stefan
Mazet, Muriel
Ziebart, Nicole
Bouyssou, Guillaume
Fouillen, Laetitia
Dupuy, Jean-William
Bonneu, Marc
Moreau, Patrick
Bringaud, Frédéric
Boshart, Michael
author_facet Allmann, Stefan
Mazet, Muriel
Ziebart, Nicole
Bouyssou, Guillaume
Fouillen, Laetitia
Dupuy, Jean-William
Bonneu, Marc
Moreau, Patrick
Bringaud, Frédéric
Boshart, Michael
author_sort Allmann, Stefan
collection PubMed
description Carbon storage is likely to enable adaptation of trypanosomes to nutritional challenges or bottlenecks during their stage development and migration in the tsetse. Lipid droplets are candidates for this function. This report shows that feeding of T. brucei with oleate results in a 4–5 fold increase in the number of lipid droplets, as quantified by confocal fluorescence microscopy and by flow cytometry of BODIPY 493/503-stained cells. The triacylglycerol (TAG) content also increased 4–5 fold, and labeled oleate is incorporated into TAG. Fatty acid carbon can thus be stored as TAG in lipid droplets under physiological growth conditions in procyclic T. brucei. β-oxidation has been suggested as a possible catabolic pathway for lipids in T. brucei. A single candidate gene, TFEα1 with coding capacity for a subunit of the trifunctional enzyme complex was identified. TFEα1 is expressed in procyclic T. brucei and present in glycosomal proteomes, Unexpectedly, a TFEα1 gene knock-out mutant still expressed wild-type levels of previously reported NADP-dependent 3-hydroxyacyl-CoA dehydrogenase activity, and therefore, another gene encodes this enzymatic activity. Homozygous Δtfeα1/Δtfeα1 null mutant cells show a normal growth rate and an unchanged glycosomal proteome in procyclic T. brucei. The decay kinetics of accumulated lipid droplets upon oleate withdrawal can be fully accounted for by the dilution effect of cell division in wild-type and Δtfeα1/Δtfeα1 cells. The absence of net catabolism of stored TAG in procyclic T. brucei, even under strictly glucose-free conditions, does not formally exclude a flux through TAG, in which biosynthesis equals catabolism. Also, the possibility remains that TAG catabolism is completely repressed by other carbon sources in culture media or developmentally activated in post-procyclic stages in the tsetse.
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spelling pubmed-42624332014-12-15 Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei Allmann, Stefan Mazet, Muriel Ziebart, Nicole Bouyssou, Guillaume Fouillen, Laetitia Dupuy, Jean-William Bonneu, Marc Moreau, Patrick Bringaud, Frédéric Boshart, Michael PLoS One Research Article Carbon storage is likely to enable adaptation of trypanosomes to nutritional challenges or bottlenecks during their stage development and migration in the tsetse. Lipid droplets are candidates for this function. This report shows that feeding of T. brucei with oleate results in a 4–5 fold increase in the number of lipid droplets, as quantified by confocal fluorescence microscopy and by flow cytometry of BODIPY 493/503-stained cells. The triacylglycerol (TAG) content also increased 4–5 fold, and labeled oleate is incorporated into TAG. Fatty acid carbon can thus be stored as TAG in lipid droplets under physiological growth conditions in procyclic T. brucei. β-oxidation has been suggested as a possible catabolic pathway for lipids in T. brucei. A single candidate gene, TFEα1 with coding capacity for a subunit of the trifunctional enzyme complex was identified. TFEα1 is expressed in procyclic T. brucei and present in glycosomal proteomes, Unexpectedly, a TFEα1 gene knock-out mutant still expressed wild-type levels of previously reported NADP-dependent 3-hydroxyacyl-CoA dehydrogenase activity, and therefore, another gene encodes this enzymatic activity. Homozygous Δtfeα1/Δtfeα1 null mutant cells show a normal growth rate and an unchanged glycosomal proteome in procyclic T. brucei. The decay kinetics of accumulated lipid droplets upon oleate withdrawal can be fully accounted for by the dilution effect of cell division in wild-type and Δtfeα1/Δtfeα1 cells. The absence of net catabolism of stored TAG in procyclic T. brucei, even under strictly glucose-free conditions, does not formally exclude a flux through TAG, in which biosynthesis equals catabolism. Also, the possibility remains that TAG catabolism is completely repressed by other carbon sources in culture media or developmentally activated in post-procyclic stages in the tsetse. Public Library of Science 2014-12-10 /pmc/articles/PMC4262433/ /pubmed/25493940 http://dx.doi.org/10.1371/journal.pone.0114628 Text en © 2014 Allmann et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Allmann, Stefan
Mazet, Muriel
Ziebart, Nicole
Bouyssou, Guillaume
Fouillen, Laetitia
Dupuy, Jean-William
Bonneu, Marc
Moreau, Patrick
Bringaud, Frédéric
Boshart, Michael
Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title_full Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title_fullStr Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title_full_unstemmed Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title_short Triacylglycerol Storage in Lipid Droplets in Procyclic Trypanosoma brucei
title_sort triacylglycerol storage in lipid droplets in procyclic trypanosoma brucei
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262433/
https://www.ncbi.nlm.nih.gov/pubmed/25493940
http://dx.doi.org/10.1371/journal.pone.0114628
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