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Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells
Panax ginseng is traditionally used as a remedy for cancer, inflammation, stress and aging, and ginsenoside-Rg5 is a major bioactive constituent of steamed ginseng. The present study aimed to evaluate whether ginsenoside-Rg5 had any marked cytotoxic, apoptotic or DNA-damaging effects in human cervic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262516/ https://www.ncbi.nlm.nih.gov/pubmed/25355274 http://dx.doi.org/10.3892/mmr.2014.2821 |
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author | LIANG, LI-DAN HE, TAO DU, TING-WEI FAN, YONG-GANG CHEN, DIAN-SEN WANG, YAN |
author_facet | LIANG, LI-DAN HE, TAO DU, TING-WEI FAN, YONG-GANG CHEN, DIAN-SEN WANG, YAN |
author_sort | LIANG, LI-DAN |
collection | PubMed |
description | Panax ginseng is traditionally used as a remedy for cancer, inflammation, stress and aging, and ginsenoside-Rg5 is a major bioactive constituent of steamed ginseng. The present study aimed to evaluate whether ginsenoside-Rg5 had any marked cytotoxic, apoptotic or DNA-damaging effects in human cervical cancer cells. Five human cervical cancer cell lines (HeLa, MS751, C33A, Me180 and HT-3) were used to investigate the cytotoxicity of ginsenoside-Rg5 using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Additionally, the effects of ginsenoside-Rg5 on the apoptosis of HeLa and MS751 cells were detected using DNA ladder assays and flow cytometry. DNA damage was assessed in the HeLa and MS751 cells using alkaline comet assays and by detection of γH2AX focus formation. The HeLa and MS751 cells were significantly more sensitive to ginsenoside-Rg5 treatment compared with the C-33A, HT-3 and Me180 cells. As expected, ginsenoside-Rg5 induced significant concentration- and time-dependent increases in apoptosis. In addition, ginsenoside-Rg5 induced significant concentration-dependent increases in the level of DNA damage compared with the negative control. Consistent with the comet assay data, the percentage of γH2AX-positive HeLa and MS751 cells also revealed that ginsenoside-Rg5 caused DNA double-strands to break in a concentration-dependent manner. In conclusion, ginsenoside-Rg5 had marked genotoxic effects in the HeLa and MS751 cells and, thus, demonstrates potential as a genotoxic or cytotoxic drug for the treatment of cervical cancer. |
format | Online Article Text |
id | pubmed-4262516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-42625162014-12-12 Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells LIANG, LI-DAN HE, TAO DU, TING-WEI FAN, YONG-GANG CHEN, DIAN-SEN WANG, YAN Mol Med Rep Articles Panax ginseng is traditionally used as a remedy for cancer, inflammation, stress and aging, and ginsenoside-Rg5 is a major bioactive constituent of steamed ginseng. The present study aimed to evaluate whether ginsenoside-Rg5 had any marked cytotoxic, apoptotic or DNA-damaging effects in human cervical cancer cells. Five human cervical cancer cell lines (HeLa, MS751, C33A, Me180 and HT-3) were used to investigate the cytotoxicity of ginsenoside-Rg5 using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Additionally, the effects of ginsenoside-Rg5 on the apoptosis of HeLa and MS751 cells were detected using DNA ladder assays and flow cytometry. DNA damage was assessed in the HeLa and MS751 cells using alkaline comet assays and by detection of γH2AX focus formation. The HeLa and MS751 cells were significantly more sensitive to ginsenoside-Rg5 treatment compared with the C-33A, HT-3 and Me180 cells. As expected, ginsenoside-Rg5 induced significant concentration- and time-dependent increases in apoptosis. In addition, ginsenoside-Rg5 induced significant concentration-dependent increases in the level of DNA damage compared with the negative control. Consistent with the comet assay data, the percentage of γH2AX-positive HeLa and MS751 cells also revealed that ginsenoside-Rg5 caused DNA double-strands to break in a concentration-dependent manner. In conclusion, ginsenoside-Rg5 had marked genotoxic effects in the HeLa and MS751 cells and, thus, demonstrates potential as a genotoxic or cytotoxic drug for the treatment of cervical cancer. D.A. Spandidos 2015-02 2014-10-30 /pmc/articles/PMC4262516/ /pubmed/25355274 http://dx.doi.org/10.3892/mmr.2014.2821 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles LIANG, LI-DAN HE, TAO DU, TING-WEI FAN, YONG-GANG CHEN, DIAN-SEN WANG, YAN Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title | Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title_full | Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title_fullStr | Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title_full_unstemmed | Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title_short | Ginsenoside-Rg5 induces apoptosis and DNA damage in human cervical cancer cells |
title_sort | ginsenoside-rg5 induces apoptosis and dna damage in human cervical cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262516/ https://www.ncbi.nlm.nih.gov/pubmed/25355274 http://dx.doi.org/10.3892/mmr.2014.2821 |
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