Cargando…
Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples
We have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA....
Autores principales: | Matranga, Christian B, Andersen, Kristian G, Winnicki, Sarah, Busby, Michele, Gladden, Adrianne D, Tewhey, Ryan, Stremlau, Matthew, Berlin, Aaron, Gire, Stephen K, England, Eleina, Moses, Lina M, Mikkelsen, Tarjei S, Odia, Ikponmwonsa, Ehiane, Philomena E, Folarin, Onikepe, Goba, Augustine, Kahn, S Humarr, Grant, Donald S, Honko, Anna, Hensley, Lisa, Happi, Christian, Garry, Robert F, Malboeuf, Christine M, Birren, Bruce W, Gnirke, Andreas, Levin, Joshua Z, Sabeti, Pardis C |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262991/ https://www.ncbi.nlm.nih.gov/pubmed/25403361 http://dx.doi.org/10.1186/s13059-014-0519-7 |
Ejemplares similares
-
Discovery of Novel Rhabdoviruses in the Blood of Healthy Individuals from West Africa
por: Stremlau, Matthew H., et al.
Publicado: (2015) -
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
por: Matranga, Christian B., et al.
Publicado: (2016) -
Empowering African genomics for infectious disease control
por: Folarin, Onikepe A, et al.
Publicado: (2014) -
Molecular Diagnostics for Lassa Fever at Irrua Specialist Teaching Hospital, Nigeria: Lessons Learnt from Two Years of Laboratory Operation
por: Asogun, Danny A., et al.
Publicado: (2012) -
Ebola Virus Epidemiology and Evolution in Nigeria
por: Folarin, Onikepe A., et al.
Publicado: (2016)