Cargando…

A thin layer angiogenesis assay: a modified basement matrix assay for assessment of endothelial cell differentiation

BACKGROUND: Basement matrices such as Matrigel™ and Geltrex™ are used in a variety of cell culture assays of anchorage-dependent differentiation including endothelial cell tube formation assays. The volumes of matrix recommended for these assays (approximately 150 μl/cm(2)) are costly, limit working...

Descripción completa

Detalles Bibliográficos
Autores principales:	Faulkner, Ashton, Purcell, Robert, Hibbert, Andrew, Latham, Sally, Thomson, Scott, Hall, Wendy L, Wheeler-Jones, Caroline, Bishop-Bailey, David
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263020/ https://www.ncbi.nlm.nih.gov/pubmed/25476021 http://dx.doi.org/10.1186/s12860-014-0041-5

Ejemplares similares

A microcarrier-based spheroid 3D invasion assay to monitor dynamic cell movement in extracellular matrix
por: Liu, Hui, et al.
Publicado: (2020)

Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures
por: Ferrer, Jordi, et al.
Publicado: (2008)

Development of a one-step embryonic stem cell-based assay for the screening of sprouting angiogenesis
por: Hermant, Bastien, et al.
Publicado: (2007)

Assaying protein palmitoylation in plants
por: Hemsley, Piers A, et al.
Publicado: (2008)

The Granzyme B ELISPOT assay: an alternative to the (51)Cr-release assay for monitoring cell-mediated cytotoxicity
por: Shafer-Weaver, Kimberly, et al.
Publicado: (2003)

High-throughput malaria serosurveillance using a one-step multiplex bead assay
por: Rogier, Eric, et al.
Publicado: (2019)

A high sensitivity assay is more accurate than a classical assay for the measurement of plasma CRP levels in endometriosis
por: Vodolazkaia, Alexandra, et al.
Publicado: (2011)

Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study
por: Buch, Karl, et al.
Publicado: (2012)

A novel fluorescent assay for sucrose transporters
por: Gora, Peter J, et al.
Publicado: (2012)

A fluorometric assay for trehalose in the picomole range
por: Carillo, Petronia, et al.
Publicado: (2013)

A seeding based cellular assay of tauopathy
por: Xu, Yin, et al.
Publicado: (2016)

CFAssay: statistical analysis of the colony formation assay
por: Braselmann, Herbert, et al.
Publicado: (2015)

Improved spectrophotometric assay for lytic polysaccharide monooxygenase
por: Breslmayr, Erik, et al.
Publicado: (2019)

A catalytically and genetically optimized β-lactamase-matrix based assay for sensitive, specific, and higher throughput analysis of native henipavirus entry characteristics
por: Wolf, Mike C, et al.
Publicado: (2009)

Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke
por: Hawkins, Kimberly E, et al.
Publicado: (2013)

A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein
por: Landeck, Natalie, et al.
Publicado: (2016)

Evaluation of Angiogenesis Assays
por: Stryker, Zachary I., et al.
Publicado: (2019)

Methods for transient assay of gene function in floral tissues
por: Shang, Yongjin, et al.
Publicado: (2007)

Counting colonies of clonogenic assays by using densitometric software
por: Niyazi, Maximilian, et al.
Publicado: (2007)

Resazurin-based assay for screening bacteria for radiation sensitivity
por: Hudman, Deborah A, et al.
Publicado: (2013)

The IN/OUT assay: a new tool to study ciliogenesis
por: Kukic, Ira, et al.
Publicado: (2016)

Colorimetric LPMO assay with direct implication for cellulolytic activity
por: Brander, Søren, et al.
Publicado: (2021)

An automated workflow to screen alkene reductases using high-throughput thin layer chromatography
por: Garabedian, Brett M., et al.
Publicado: (2020)

A novel assay for analysis of the regulation of the function of human osteoclasts
por: Kirstein, Barrie, et al.
Publicado: (2006)

New low-viscosity overlay medium for viral plaque assays
por: Matrosovich, Mikhail, et al.
Publicado: (2006)

Development and Validation of a HPV-32 Specific PCR Assay
por: Herrel, Nicholas R, et al.
Publicado: (2009)

Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays
por: Wilson, Danny W, et al.
Publicado: (2010)

Sample-ready multiplex qPCR assay for detection of malaria
por: Kamau, Edwin, et al.
Publicado: (2014)

New molecular settings to support in vivo anti-malarial assays
por: Bahamontes-Rosa, Noemí, et al.
Publicado: (2016)

Comparison of three PCR-based assays for SNP genotyping in plants
por: Broccanello, Chiara, et al.
Publicado: (2018)

A bacterial assay for rapid screening of IAA catabolic enzymes
por: Brunoni, Federica, et al.
Publicado: (2019)

A rapid assay for assessing bacterial effects on Arabidopsis thermotolerance
por: Lee, Jun Hyung, et al.
Publicado: (2023)

A fluorescence-based assay for Trichomonas vaginalis drug screening
por: Chen, Qianqian, et al.
Publicado: (2023)

Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
por: Mucker, Eric M., et al.
Publicado: (2017)

High-throughput kinase assays with protein substrates using fluorescent polymer superquenching
por: Rininsland, Frauke, et al.
Publicado: (2005)

Use of thin-layer chromatography to detect counterfeit sulfadoxine/pyrimethamine tablets with the wrong active ingredient in Malawi
por: Khuluza, Felix, et al.
Publicado: (2016)

Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay
por: Susa, Mira, et al.
Publicado: (2004)

High throughput RNAi assay optimization using adherent cell cytometry
por: Nabzdyk, Christoph S, et al.
Publicado: (2011)

Evaluation and optimization of membrane feeding compared to direct feeding as an assay for infectivity
por: Diallo, Mouctar, et al.
Publicado: (2008)

Precursor uptake assays and metabolic analyses in isolated tomato fruit chromoplasts
por: Angaman, Djédoux Maxime, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_sbs6ro21sfkjodl483np38oj9t