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Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat

BACKGROUND: Species belonging to the genus Aegilops L. are an important source of genetic material for expanding genetic variability of wheat. Ae. triuncialis is an allotetraploid in this genus which was originated from hybridization of Ae. umbellulata and Ae. markgrafii (Greuter) Hammer. Although t...

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Autores principales: Mirzaghaderi, Ghader, Houben, Andreas, Badaeva, Ekaterina D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263106/
https://www.ncbi.nlm.nih.gov/pubmed/25505933
http://dx.doi.org/10.1186/s13039-014-0091-6
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author Mirzaghaderi, Ghader
Houben, Andreas
Badaeva, Ekaterina D
author_facet Mirzaghaderi, Ghader
Houben, Andreas
Badaeva, Ekaterina D
author_sort Mirzaghaderi, Ghader
collection PubMed
description BACKGROUND: Species belonging to the genus Aegilops L. are an important source of genetic material for expanding genetic variability of wheat. Ae. triuncialis is an allotetraploid in this genus which was originated from hybridization of Ae. umbellulata and Ae. markgrafii (Greuter) Hammer. Although the Ae. triuncialis karyotype was thoroughly examined by conventional chromosome staining and Giemsa C-banding, it is still poorly characterized using FISH markers. The objective of this study was to test the fluorescence in situ hybridization (FISH) patterns of Ae. triuncialis (2n = 4x = 28, C(t)C(t)U(t)U(t)) chromosomes using different repetitive sequences and to compare the produced patterns to the chromosomes of its diploid ancestors, with the aim of establishing a generalized Ae. triuncialis idiogram and detection of Aegilops chromosomes in the background of wheat. RESULTS: The probes pSc119.2-1, pTa535-1, pAs1-1, (CTT)(10) and the 45S rDNA clone from wheat (pTa71) were hybridized to chromosomes of Ae. triuncialis and compared with its diploid progenitors (Ae. umbellulata Zhuk., 2n = 2x = 14, UU and Ae. markgrafii (Greuter) Hammer, 2n = 2x = 14, CC) and Ae. cylindrica Host. (2n = 4x = 28, D(c)D(c)C(c)C(c)), another tetraploid species containing the C-genome. Ae. cylindrica was further analyzed by genomic in situ hybridization (GISH) using C genome probe in order to identify any possible translocation. CONCLUSIONS: In general, FISH patterns of the U(t)- and C(t)-genome chromosomes of Ae. triuncialis were similar to those of U- and C-genome chromosomes of the diploid progenitor species Ae. umbellulata and Ae. markgrafii respectively, although some differences were observed. Two major 45S rDNA loci were revealed in the short arm of chromosomes A and C, of the C(t) genome which correspond to homoeologous groups 1 and 5 respectively. Minor 45S rDNA loci were mapped on the short arm of chromosomes 1U(t) and 5U(t). GISH analysis revealed three different non-reciprocal homologous or heterologous translocations between C(c) and D(c) chromosomes in all studied accessions of Ae. cylindrica. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13039-014-0091-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-42631062014-12-12 Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat Mirzaghaderi, Ghader Houben, Andreas Badaeva, Ekaterina D Mol Cytogenet Research BACKGROUND: Species belonging to the genus Aegilops L. are an important source of genetic material for expanding genetic variability of wheat. Ae. triuncialis is an allotetraploid in this genus which was originated from hybridization of Ae. umbellulata and Ae. markgrafii (Greuter) Hammer. Although the Ae. triuncialis karyotype was thoroughly examined by conventional chromosome staining and Giemsa C-banding, it is still poorly characterized using FISH markers. The objective of this study was to test the fluorescence in situ hybridization (FISH) patterns of Ae. triuncialis (2n = 4x = 28, C(t)C(t)U(t)U(t)) chromosomes using different repetitive sequences and to compare the produced patterns to the chromosomes of its diploid ancestors, with the aim of establishing a generalized Ae. triuncialis idiogram and detection of Aegilops chromosomes in the background of wheat. RESULTS: The probes pSc119.2-1, pTa535-1, pAs1-1, (CTT)(10) and the 45S rDNA clone from wheat (pTa71) were hybridized to chromosomes of Ae. triuncialis and compared with its diploid progenitors (Ae. umbellulata Zhuk., 2n = 2x = 14, UU and Ae. markgrafii (Greuter) Hammer, 2n = 2x = 14, CC) and Ae. cylindrica Host. (2n = 4x = 28, D(c)D(c)C(c)C(c)), another tetraploid species containing the C-genome. Ae. cylindrica was further analyzed by genomic in situ hybridization (GISH) using C genome probe in order to identify any possible translocation. CONCLUSIONS: In general, FISH patterns of the U(t)- and C(t)-genome chromosomes of Ae. triuncialis were similar to those of U- and C-genome chromosomes of the diploid progenitor species Ae. umbellulata and Ae. markgrafii respectively, although some differences were observed. Two major 45S rDNA loci were revealed in the short arm of chromosomes A and C, of the C(t) genome which correspond to homoeologous groups 1 and 5 respectively. Minor 45S rDNA loci were mapped on the short arm of chromosomes 1U(t) and 5U(t). GISH analysis revealed three different non-reciprocal homologous or heterologous translocations between C(c) and D(c) chromosomes in all studied accessions of Ae. cylindrica. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13039-014-0091-6) contains supplementary material, which is available to authorized users. BioMed Central 2014-12-02 /pmc/articles/PMC4263106/ /pubmed/25505933 http://dx.doi.org/10.1186/s13039-014-0091-6 Text en © Mirzaghaderi et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Mirzaghaderi, Ghader
Houben, Andreas
Badaeva, Ekaterina D
Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title_full Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title_fullStr Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title_full_unstemmed Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title_short Molecular-cytogenetic analysis of Aegilops triuncialis and identification of its chromosomes in the background of wheat
title_sort molecular-cytogenetic analysis of aegilops triuncialis and identification of its chromosomes in the background of wheat
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263106/
https://www.ncbi.nlm.nih.gov/pubmed/25505933
http://dx.doi.org/10.1186/s13039-014-0091-6
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