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Serotonin receptor 3A controls interneuron migration into the neocortex

Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic s...

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Autores principales: Murthy, Sahana, Niquille, Mathieu, Hurni, Nicolas, Limoni, Greta, Frazer, Sarah, Chameau, Pascal, van Hooft, Johannes A., Vitalis, Tania, Dayer, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263148/
https://www.ncbi.nlm.nih.gov/pubmed/25409778
http://dx.doi.org/10.1038/ncomms6524
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author Murthy, Sahana
Niquille, Mathieu
Hurni, Nicolas
Limoni, Greta
Frazer, Sarah
Chameau, Pascal
van Hooft, Johannes A.
Vitalis, Tania
Dayer, Alexandre
author_facet Murthy, Sahana
Niquille, Mathieu
Hurni, Nicolas
Limoni, Greta
Frazer, Sarah
Chameau, Pascal
van Hooft, Johannes A.
Vitalis, Tania
Dayer, Alexandre
author_sort Murthy, Sahana
collection PubMed
description Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic serotonin receptor 3A (5-HT(3A)R) is specifically expressed in CGE-derived migrating interneurons and upregulated while they invade the developing cortex. Functional investigations using calcium imaging, electrophysiological recordings and migration assays indicate that CGE-derived INs increase their response to 5-HT(3A)R activation during the late phase of cortical plate invasion. Using genetic loss-of-function approaches and in vivo grafts, we further demonstrate that the 5-HT(3A)R is cell autonomously required for the migration and proper positioning of reelin-expressing CGE-derived INs in the neocortex. Our findings reveal a requirement for a serotonin receptor in controlling the migration and laminar positioning of a specific subtype of cortical IN.
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spelling pubmed-42631482014-12-16 Serotonin receptor 3A controls interneuron migration into the neocortex Murthy, Sahana Niquille, Mathieu Hurni, Nicolas Limoni, Greta Frazer, Sarah Chameau, Pascal van Hooft, Johannes A. Vitalis, Tania Dayer, Alexandre Nat Commun Article Neuronal excitability has been shown to control the migration and cortical integration of reelin-expressing cortical interneurons (INs) arising from the caudal ganglionic eminence (CGE), supporting the possibility that neurotransmitters could regulate this process. Here we show that the ionotropic serotonin receptor 3A (5-HT(3A)R) is specifically expressed in CGE-derived migrating interneurons and upregulated while they invade the developing cortex. Functional investigations using calcium imaging, electrophysiological recordings and migration assays indicate that CGE-derived INs increase their response to 5-HT(3A)R activation during the late phase of cortical plate invasion. Using genetic loss-of-function approaches and in vivo grafts, we further demonstrate that the 5-HT(3A)R is cell autonomously required for the migration and proper positioning of reelin-expressing CGE-derived INs in the neocortex. Our findings reveal a requirement for a serotonin receptor in controlling the migration and laminar positioning of a specific subtype of cortical IN. Nature Pub. Group 2014-11-20 /pmc/articles/PMC4263148/ /pubmed/25409778 http://dx.doi.org/10.1038/ncomms6524 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Murthy, Sahana
Niquille, Mathieu
Hurni, Nicolas
Limoni, Greta
Frazer, Sarah
Chameau, Pascal
van Hooft, Johannes A.
Vitalis, Tania
Dayer, Alexandre
Serotonin receptor 3A controls interneuron migration into the neocortex
title Serotonin receptor 3A controls interneuron migration into the neocortex
title_full Serotonin receptor 3A controls interneuron migration into the neocortex
title_fullStr Serotonin receptor 3A controls interneuron migration into the neocortex
title_full_unstemmed Serotonin receptor 3A controls interneuron migration into the neocortex
title_short Serotonin receptor 3A controls interneuron migration into the neocortex
title_sort serotonin receptor 3a controls interneuron migration into the neocortex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263148/
https://www.ncbi.nlm.nih.gov/pubmed/25409778
http://dx.doi.org/10.1038/ncomms6524
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