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Yeast Pif1 Accelerates Annealing of Complementary DNA Strands

[Image: see text] Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as those that generate a duple...

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Autores principales: Ramanagoudr-Bhojappa, Ramanagouda, Byrd, Alicia K., Dahl, Christopher, Raney, Kevin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263423/
https://www.ncbi.nlm.nih.gov/pubmed/25393406
http://dx.doi.org/10.1021/bi500746v
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author Ramanagoudr-Bhojappa, Ramanagouda
Byrd, Alicia K.
Dahl, Christopher
Raney, Kevin D.
author_facet Ramanagoudr-Bhojappa, Ramanagouda
Byrd, Alicia K.
Dahl, Christopher
Raney, Kevin D.
author_sort Ramanagoudr-Bhojappa, Ramanagouda
collection PubMed
description [Image: see text] Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as those that generate a duplex product with a single-stranded overhang relative to a blunt end duplex. Importantly, we show that Pif1 can anneal DNA in the presence of ATP and Mg(2+). Pif1-mediated annealing also occurs in the presence of single-stranded DNA binding proteins. Additionally, we show that partial duplex substrates with 3′-single-stranded overhangs such as those generated during double-strand break repair can be annealed by Pif1.
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spelling pubmed-42634232015-11-13 Yeast Pif1 Accelerates Annealing of Complementary DNA Strands Ramanagoudr-Bhojappa, Ramanagouda Byrd, Alicia K. Dahl, Christopher Raney, Kevin D. Biochemistry [Image: see text] Pif1 is a helicase involved in the maintenance of nuclear and mitochondrial genomes in eukaryotes. Here we report a new activity of Saccharomyces cerevisiae Pif1, annealing of complementary DNA strands. We identified preferred substrates for annealing as those that generate a duplex product with a single-stranded overhang relative to a blunt end duplex. Importantly, we show that Pif1 can anneal DNA in the presence of ATP and Mg(2+). Pif1-mediated annealing also occurs in the presence of single-stranded DNA binding proteins. Additionally, we show that partial duplex substrates with 3′-single-stranded overhangs such as those generated during double-strand break repair can be annealed by Pif1. American Chemical Society 2014-11-13 2014-12-09 /pmc/articles/PMC4263423/ /pubmed/25393406 http://dx.doi.org/10.1021/bi500746v Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Ramanagoudr-Bhojappa, Ramanagouda
Byrd, Alicia K.
Dahl, Christopher
Raney, Kevin D.
Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title_full Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title_fullStr Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title_full_unstemmed Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title_short Yeast Pif1 Accelerates Annealing of Complementary DNA Strands
title_sort yeast pif1 accelerates annealing of complementary dna strands
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263423/
https://www.ncbi.nlm.nih.gov/pubmed/25393406
http://dx.doi.org/10.1021/bi500746v
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