Cargando…
An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos
Cytokinesis in animal cells depends on spindle-derived spatial cues that culminate in Rho activation, and thereby actomyosin assembly, in a narrow equatorial band. Although the nature, origin, and variety of such cues have long been obscure, one component is certainly the Rho activator Ect2. Here we...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263448/ https://www.ncbi.nlm.nih.gov/pubmed/25298401 http://dx.doi.org/10.1091/mbc.E14-04-0859 |
_version_ | 1782348572158590976 |
---|---|
author | Su, Kuan-Chung Bement, William M. Petronczki, Mark von Dassow, George |
author_facet | Su, Kuan-Chung Bement, William M. Petronczki, Mark von Dassow, George |
author_sort | Su, Kuan-Chung |
collection | PubMed |
description | Cytokinesis in animal cells depends on spindle-derived spatial cues that culminate in Rho activation, and thereby actomyosin assembly, in a narrow equatorial band. Although the nature, origin, and variety of such cues have long been obscure, one component is certainly the Rho activator Ect2. Here we describe the behavior and function of Ect2 in echinoderm embryos, showing that Ect2 migrates from spindle midzone to astral microtubules in anaphase and that Ect2 shapes the pattern of Rho activation in incipient furrows. Our key finding is that Ect2 and its binding partner Cyk4 accumulate not only at normal furrows, but also at furrows that form in the absence of associated spindle, midzone, or chromosomes. In all these cases, the cell assembles essentially the same cytokinetic signaling ensemble—opposed astral microtubules decorated with Ect2 and Cyk4. We conclude that if multiple signals contribute to furrow induction in echinoderm embryos, they likely converge on the same signaling ensemble on an analogous cytoskeletal scaffold. |
format | Online Article Text |
id | pubmed-4263448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42634482015-03-02 An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos Su, Kuan-Chung Bement, William M. Petronczki, Mark von Dassow, George Mol Biol Cell Articles Cytokinesis in animal cells depends on spindle-derived spatial cues that culminate in Rho activation, and thereby actomyosin assembly, in a narrow equatorial band. Although the nature, origin, and variety of such cues have long been obscure, one component is certainly the Rho activator Ect2. Here we describe the behavior and function of Ect2 in echinoderm embryos, showing that Ect2 migrates from spindle midzone to astral microtubules in anaphase and that Ect2 shapes the pattern of Rho activation in incipient furrows. Our key finding is that Ect2 and its binding partner Cyk4 accumulate not only at normal furrows, but also at furrows that form in the absence of associated spindle, midzone, or chromosomes. In all these cases, the cell assembles essentially the same cytokinetic signaling ensemble—opposed astral microtubules decorated with Ect2 and Cyk4. We conclude that if multiple signals contribute to furrow induction in echinoderm embryos, they likely converge on the same signaling ensemble on an analogous cytoskeletal scaffold. The American Society for Cell Biology 2014-12-15 /pmc/articles/PMC4263448/ /pubmed/25298401 http://dx.doi.org/10.1091/mbc.E14-04-0859 Text en © 2014 Su et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Su, Kuan-Chung Bement, William M. Petronczki, Mark von Dassow, George An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title | An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title_full | An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title_fullStr | An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title_full_unstemmed | An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title_short | An astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
title_sort | astral simulacrum of the central spindle accounts for normal, spindle-less, and anucleate cytokinesis in echinoderm embryos |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263448/ https://www.ncbi.nlm.nih.gov/pubmed/25298401 http://dx.doi.org/10.1091/mbc.E14-04-0859 |
work_keys_str_mv | AT sukuanchung anastralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT bementwilliamm anastralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT petronczkimark anastralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT vondassowgeorge anastralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT sukuanchung astralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT bementwilliamm astralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT petronczkimark astralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos AT vondassowgeorge astralsimulacrumofthecentralspindleaccountsfornormalspindlelessandanucleatecytokinesisinechinodermembryos |