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Enzyme-Gelatin Electrochemical Biosensors: Scaling Down
In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263544/ https://www.ncbi.nlm.nih.gov/pubmed/25585635 http://dx.doi.org/10.3390/bios2010101 |
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author | Wael, Karolien De Belder, Stijn De Pilehvar, Sanaz Steenberge, Geert Van Herrebout, Wouter Heering, Hendrik A. |
author_facet | Wael, Karolien De Belder, Stijn De Pilehvar, Sanaz Steenberge, Geert Van Herrebout, Wouter Heering, Hendrik A. |
author_sort | Wael, Karolien De |
collection | PubMed |
description | In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix. |
format | Online Article Text |
id | pubmed-4263544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42635442015-01-13 Enzyme-Gelatin Electrochemical Biosensors: Scaling Down Wael, Karolien De Belder, Stijn De Pilehvar, Sanaz Steenberge, Geert Van Herrebout, Wouter Heering, Hendrik A. Biosensors (Basel) Article In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a drop dried and a spin coated layer was prepared. On scaling down, a transition from diffusion controlled reactions towards adsorption controlled reactions is observed. Compared to a drop dried electrode, a spin coated electrode showed a more stable electrochemical behavior. Next to HHC, we also incorporated catalase in a spin coated gelatin matrix immobilized on a glassy carbon electrode. By spincoating, highly uniform sub micrometer layers of biocompatible matrices can be constructed. A full electrochemical study and characterization of the modified surfaces has been carried out. It was clear that in the case of catalase, gluteraldehyde addition was needed to prevent leaking of the catalase from the gelatin matrix. MDPI 2012-03-15 /pmc/articles/PMC4263544/ /pubmed/25585635 http://dx.doi.org/10.3390/bios2010101 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Wael, Karolien De Belder, Stijn De Pilehvar, Sanaz Steenberge, Geert Van Herrebout, Wouter Heering, Hendrik A. Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title | Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title_full | Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title_fullStr | Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title_full_unstemmed | Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title_short | Enzyme-Gelatin Electrochemical Biosensors: Scaling Down |
title_sort | enzyme-gelatin electrochemical biosensors: scaling down |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263544/ https://www.ncbi.nlm.nih.gov/pubmed/25585635 http://dx.doi.org/10.3390/bios2010101 |
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