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Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors

An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platfo...

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Detalles Bibliográficos
Autores principales: McGraw, Shannon K., Alocilja, Evangelyn, Senecal, Andre, Senecal, Kris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263555/
https://www.ncbi.nlm.nih.gov/pubmed/25586036
http://dx.doi.org/10.3390/bios2040465
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author McGraw, Shannon K.
Alocilja, Evangelyn
Senecal, Andre
Senecal, Kris
author_facet McGraw, Shannon K.
Alocilja, Evangelyn
Senecal, Andre
Senecal, Kris
author_sort McGraw, Shannon K.
collection PubMed
description An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems.
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spelling pubmed-42635552015-01-13 Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors McGraw, Shannon K. Alocilja, Evangelyn Senecal, Andre Senecal, Kris Biosensors (Basel) Article An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems. MDPI 2012-11-29 /pmc/articles/PMC4263555/ /pubmed/25586036 http://dx.doi.org/10.3390/bios2040465 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
McGraw, Shannon K.
Alocilja, Evangelyn
Senecal, Andre
Senecal, Kris
Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title_full Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title_fullStr Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title_full_unstemmed Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title_short Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
title_sort synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263555/
https://www.ncbi.nlm.nih.gov/pubmed/25586036
http://dx.doi.org/10.3390/bios2040465
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