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Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors
An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platfo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263555/ https://www.ncbi.nlm.nih.gov/pubmed/25586036 http://dx.doi.org/10.3390/bios2040465 |
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author | McGraw, Shannon K. Alocilja, Evangelyn Senecal, Andre Senecal, Kris |
author_facet | McGraw, Shannon K. Alocilja, Evangelyn Senecal, Andre Senecal, Kris |
author_sort | McGraw, Shannon K. |
collection | PubMed |
description | An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems. |
format | Online Article Text |
id | pubmed-4263555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42635552015-01-13 Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors McGraw, Shannon K. Alocilja, Evangelyn Senecal, Andre Senecal, Kris Biosensors (Basel) Article An electrotextile with a biosensing focus composed of conductive polymer coated microfibers that contain functional attachment sites for biorecognition elements was developed. Experiments were conducted to select a compound with a pendant functional group for inclusion in the polymer, a fiber platform, and polymerization solvent. The effects of dopant inclusion and post-polymerization wash steps were also analyzed. Finally, the successful attachment of avidin, which was then used to capture biotin, to the electrotextile was achieved. The initial results show a nonwoven fiber matrix can be successfully coated in a conductive, functionalized polymer while still maintaining surface area and fiber durability. A polypropylene fiber platform with a conductive polypyrrole coating using iron (III) chloride as an oxidant, water as a solvent, and 5-sulfosalicylic acid as a dopant exhibited the best coating consistency, material durability, and lowest resistance. Biological attachment of avidin was achieved on the fibers through the inclusion of a carboxyl functional group via 3-thiopheneacetic acid in the monomer. The immobilized avidin was then successfully used to capture biotin. This was confirmed through the use of fluorescent quantum dots and confocal microscopy. A preliminary electrochemical experiment using avidin for biotin detection was conducted. This technology will be extremely useful in the formation of electrotextiles for use in biosensor systems. MDPI 2012-11-29 /pmc/articles/PMC4263555/ /pubmed/25586036 http://dx.doi.org/10.3390/bios2040465 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article McGraw, Shannon K. Alocilja, Evangelyn Senecal, Andre Senecal, Kris Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title | Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title_full | Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title_fullStr | Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title_full_unstemmed | Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title_short | Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors |
title_sort | synthesis of a functionalized polypyrrole coated electrotextile for use in biosensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263555/ https://www.ncbi.nlm.nih.gov/pubmed/25586036 http://dx.doi.org/10.3390/bios2040465 |
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