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Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage

This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun...

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Detalles Bibliográficos
Autores principales: Dai, Minhui, Jin, Shengquan, Nugen, Sam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263557/
https://www.ncbi.nlm.nih.gov/pubmed/25586029
http://dx.doi.org/10.3390/bios2040388
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author Dai, Minhui
Jin, Shengquan
Nugen, Sam R.
author_facet Dai, Minhui
Jin, Shengquan
Nugen, Sam R.
author_sort Dai, Minhui
collection PubMed
description This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3′,5,5′-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform.
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spelling pubmed-42635572015-01-13 Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage Dai, Minhui Jin, Shengquan Nugen, Sam R. Biosensors (Basel) Communication This work demonstrates the ability to electrospin reagents into water-soluble nanofibers resulting in a stable on-chip enzyme storage format. Polyvinylpyrrolidone (PVP) nanofibers were spun with incorporation of the enzyme horseradish peroxidase (HRP). Scanning electron microscopy (SEM) of the spun nanofibers was used to confirm the non-woven structure which had an average diameter of 155 ± 34 nm. The HRP containing fibers were tested for their change in activity following electrospinning and during storage. A colorimetric assay was used to characterize the activity of HRP by reaction with the nanofiber mats in a microtiter plate and monitoring the change in absorption over time. Immediately following electrospinning, the activity peak for the HRP decreased by approximately 20%. After a storage study over 280 days, 40% of the activity remained. In addition to activity, the fibers were observed to solubilize in the microfluidic chamber. The chromogenic 3,3′,5,5′-tetramethylbenzidine solution reacted immediately with the fibers as they passed through a microfluidic channel. The ability to store enzymes and other reagents on-chip in a rapidly dispersible format could reduce the assay steps required of an operator to perform. MDPI 2012-10-08 /pmc/articles/PMC4263557/ /pubmed/25586029 http://dx.doi.org/10.3390/bios2040388 Text en © 2012 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Dai, Minhui
Jin, Shengquan
Nugen, Sam R.
Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title_full Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title_fullStr Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title_full_unstemmed Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title_short Water-Soluble Electrospun Nanofibers as a Method for On-Chip Reagent Storage
title_sort water-soluble electrospun nanofibers as a method for on-chip reagent storage
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263557/
https://www.ncbi.nlm.nih.gov/pubmed/25586029
http://dx.doi.org/10.3390/bios2040388
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