Cargando…

Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface

Lab-on-a-chip immuno assays utilizing superparamagnetic beads as labels suffer from the fact that the majority of beads pass the sensing area without contacting the sensor surface. Different solutions, employing magnetic forces, ultrasonic standing waves, or hydrodynamic effects have been found over...

Descripción completa

Detalles Bibliográficos
Autores principales: Eickenberg, Bernhard, Meyer, Judith, Helmich, Lars, Kappe, Daniel, Auge, Alexander, Weddemann, Alexander, Wittbracht, Frank, Hütten, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263578/
https://www.ncbi.nlm.nih.gov/pubmed/25586262
http://dx.doi.org/10.3390/bios3030327
_version_ 1782348601259720704
author Eickenberg, Bernhard
Meyer, Judith
Helmich, Lars
Kappe, Daniel
Auge, Alexander
Weddemann, Alexander
Wittbracht, Frank
Hütten, Andreas
author_facet Eickenberg, Bernhard
Meyer, Judith
Helmich, Lars
Kappe, Daniel
Auge, Alexander
Weddemann, Alexander
Wittbracht, Frank
Hütten, Andreas
author_sort Eickenberg, Bernhard
collection PubMed
description Lab-on-a-chip immuno assays utilizing superparamagnetic beads as labels suffer from the fact that the majority of beads pass the sensing area without contacting the sensor surface. Different solutions, employing magnetic forces, ultrasonic standing waves, or hydrodynamic effects have been found over the past decades. The first category uses magnetic forces, created by on-chip conducting lines to attract beads towards the sensor surface. Modifications of the magnetic landscape allow for additional transport and separation of different bead species. The hydrodynamic approach uses changes in the channel geometry to enhance the capture volume. In acoustofluidics, ultrasonic standing waves force µm-sized particles onto a surface through radiation forces. As these approaches have their disadvantages, a new sensor concept that circumvents these problems is suggested. This concept is based on the granular giant magnetoresistance (GMR) effect that can be found in gels containing magnetic nanoparticles. The proposed design could be realized in the shape of paper-based test strips printed with gel-based GMR sensors.
format Online
Article
Text
id pubmed-4263578
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42635782015-01-13 Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface Eickenberg, Bernhard Meyer, Judith Helmich, Lars Kappe, Daniel Auge, Alexander Weddemann, Alexander Wittbracht, Frank Hütten, Andreas Biosensors (Basel) Review Lab-on-a-chip immuno assays utilizing superparamagnetic beads as labels suffer from the fact that the majority of beads pass the sensing area without contacting the sensor surface. Different solutions, employing magnetic forces, ultrasonic standing waves, or hydrodynamic effects have been found over the past decades. The first category uses magnetic forces, created by on-chip conducting lines to attract beads towards the sensor surface. Modifications of the magnetic landscape allow for additional transport and separation of different bead species. The hydrodynamic approach uses changes in the channel geometry to enhance the capture volume. In acoustofluidics, ultrasonic standing waves force µm-sized particles onto a surface through radiation forces. As these approaches have their disadvantages, a new sensor concept that circumvents these problems is suggested. This concept is based on the granular giant magnetoresistance (GMR) effect that can be found in gels containing magnetic nanoparticles. The proposed design could be realized in the shape of paper-based test strips printed with gel-based GMR sensors. MDPI 2013-09-17 /pmc/articles/PMC4263578/ /pubmed/25586262 http://dx.doi.org/10.3390/bios3030327 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Eickenberg, Bernhard
Meyer, Judith
Helmich, Lars
Kappe, Daniel
Auge, Alexander
Weddemann, Alexander
Wittbracht, Frank
Hütten, Andreas
Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title_full Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title_fullStr Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title_full_unstemmed Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title_short Lab-on-a-Chip Magneto-Immunoassays: How to Ensure Contact between Superparamagnetic Beads and the Sensor Surface
title_sort lab-on-a-chip magneto-immunoassays: how to ensure contact between superparamagnetic beads and the sensor surface
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263578/
https://www.ncbi.nlm.nih.gov/pubmed/25586262
http://dx.doi.org/10.3390/bios3030327
work_keys_str_mv AT eickenbergbernhard labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT meyerjudith labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT helmichlars labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT kappedaniel labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT augealexander labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT weddemannalexander labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT wittbrachtfrank labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface
AT huttenandreas labonachipmagnetoimmunoassayshowtoensurecontactbetweensuperparamagneticbeadsandthesensorsurface