Cargando…
Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design
This report describes novel perfluorocarbon (PFC) nanoemulsions designed to improve ex vivo cell labeling for (19)F magnetic resonance imaging (MRI). (19)F MRI is a powerful non-invasive technique for monitoring cells of the immune system in vivo, where cells are labeled ex vivo with PFC nanoemulsio...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263580/ https://www.ncbi.nlm.nih.gov/pubmed/25586263 http://dx.doi.org/10.3390/bios3030341 |
_version_ | 1782348601717948416 |
---|---|
author | Patel, Sravan K. Williams, Jonathan Janjic, Jelena M. |
author_facet | Patel, Sravan K. Williams, Jonathan Janjic, Jelena M. |
author_sort | Patel, Sravan K. |
collection | PubMed |
description | This report describes novel perfluorocarbon (PFC) nanoemulsions designed to improve ex vivo cell labeling for (19)F magnetic resonance imaging (MRI). (19)F MRI is a powerful non-invasive technique for monitoring cells of the immune system in vivo, where cells are labeled ex vivo with PFC nanoemulsions in cell culture. The quality of (19)F MRI is directly affected by the quality of ex vivo PFC cell labeling. When co-cultured with cells for longer periods of time, nanoemulsions tend to settle due to high specific weight of PFC oils (1.5–2.0 g/mL). This in turn can decrease efficacy of excess nanoemulsion removal and reliability of the cell labeling in vitro. To solve this problem, novel PFC nanoemulsions are reported which demonstrate lack of sedimentation and high stability under cell labeling conditions. They are monodisperse, have small droplet size (~130 nm) and low polydispersity (<0.15), show a single peak in the (19)F nuclear magnetic resonance spectrum at −71.4 ppm and possess high fluorine content. The droplet size and polydispersity remained unchanged after 160 days of follow up at three temperatures (4, 25 and 37 °C). Further, stressors such as elevated temperature in the presence of cells, and centrifugation, did not affect the nanoemulsion droplet size and polydispersity. Detailed synthetic methodology and in vitro testing for these new PFC nanoemulsions is presented. |
format | Online Article Text |
id | pubmed-4263580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-42635802015-01-13 Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design Patel, Sravan K. Williams, Jonathan Janjic, Jelena M. Biosensors (Basel) Article This report describes novel perfluorocarbon (PFC) nanoemulsions designed to improve ex vivo cell labeling for (19)F magnetic resonance imaging (MRI). (19)F MRI is a powerful non-invasive technique for monitoring cells of the immune system in vivo, where cells are labeled ex vivo with PFC nanoemulsions in cell culture. The quality of (19)F MRI is directly affected by the quality of ex vivo PFC cell labeling. When co-cultured with cells for longer periods of time, nanoemulsions tend to settle due to high specific weight of PFC oils (1.5–2.0 g/mL). This in turn can decrease efficacy of excess nanoemulsion removal and reliability of the cell labeling in vitro. To solve this problem, novel PFC nanoemulsions are reported which demonstrate lack of sedimentation and high stability under cell labeling conditions. They are monodisperse, have small droplet size (~130 nm) and low polydispersity (<0.15), show a single peak in the (19)F nuclear magnetic resonance spectrum at −71.4 ppm and possess high fluorine content. The droplet size and polydispersity remained unchanged after 160 days of follow up at three temperatures (4, 25 and 37 °C). Further, stressors such as elevated temperature in the presence of cells, and centrifugation, did not affect the nanoemulsion droplet size and polydispersity. Detailed synthetic methodology and in vitro testing for these new PFC nanoemulsions is presented. MDPI 2013-09-23 /pmc/articles/PMC4263580/ /pubmed/25586263 http://dx.doi.org/10.3390/bios3030341 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Patel, Sravan K. Williams, Jonathan Janjic, Jelena M. Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title | Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title_full | Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title_fullStr | Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title_full_unstemmed | Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title_short | Cell Labeling for (19)F MRI: New and Improved Approach to Perfluorocarbon Nanoemulsion Design |
title_sort | cell labeling for (19)f mri: new and improved approach to perfluorocarbon nanoemulsion design |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263580/ https://www.ncbi.nlm.nih.gov/pubmed/25586263 http://dx.doi.org/10.3390/bios3030341 |
work_keys_str_mv | AT patelsravank celllabelingfor19fmrinewandimprovedapproachtoperfluorocarbonnanoemulsiondesign AT williamsjonathan celllabelingfor19fmrinewandimprovedapproachtoperfluorocarbonnanoemulsiondesign AT janjicjelenam celllabelingfor19fmrinewandimprovedapproachtoperfluorocarbonnanoemulsiondesign |