Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence

We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent ass...

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Detalles Bibliográficos
Autores principales: Winterflood, Christian M., Ruckstuhl, Thomas, Seeger, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263590/
https://www.ncbi.nlm.nih.gov/pubmed/25587401
http://dx.doi.org/10.3390/bios3010108
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author Winterflood, Christian M.
Ruckstuhl, Thomas
Seeger, Stefan
author_facet Winterflood, Christian M.
Ruckstuhl, Thomas
Seeger, Stefan
author_sort Winterflood, Christian M.
collection PubMed
description We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
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spelling pubmed-42635902015-01-13 Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence Winterflood, Christian M. Ruckstuhl, Thomas Seeger, Stefan Biosensors (Basel) Article We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements. MDPI 2013-02-08 /pmc/articles/PMC4263590/ /pubmed/25587401 http://dx.doi.org/10.3390/bios3010108 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Winterflood, Christian M.
Ruckstuhl, Thomas
Seeger, Stefan
Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title_full Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title_fullStr Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title_full_unstemmed Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title_short Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
title_sort fast and sensitive interferon-γ assay using supercritical angle fluorescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263590/
https://www.ncbi.nlm.nih.gov/pubmed/25587401
http://dx.doi.org/10.3390/bios3010108
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