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Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal miRNAs isolated from human bone marrow-derived mesenchymal stem cells (BMSCs) culture were profiled using miRNA arrays containing probes for 894 human matured miRNAs. Seventy-nine miRNAs (∼8.84%) cou...

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Autores principales: Xu, Ji-Feng, Yang, Guang-hai, Pan, Xiao-Hong, Zhang, Shui-Jun, Zhao, Chen, Qiu, Bin-Song, Gu, Hai-Feng, Hong, Jian-Fei, Cao, Li, Chen, Yu, Xia, Bing, Bi, Qin, Wang, Ya-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263734/
https://www.ncbi.nlm.nih.gov/pubmed/25503309
http://dx.doi.org/10.1371/journal.pone.0114627
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author Xu, Ji-Feng
Yang, Guang-hai
Pan, Xiao-Hong
Zhang, Shui-Jun
Zhao, Chen
Qiu, Bin-Song
Gu, Hai-Feng
Hong, Jian-Fei
Cao, Li
Chen, Yu
Xia, Bing
Bi, Qin
Wang, Ya-Ping
author_facet Xu, Ji-Feng
Yang, Guang-hai
Pan, Xiao-Hong
Zhang, Shui-Jun
Zhao, Chen
Qiu, Bin-Song
Gu, Hai-Feng
Hong, Jian-Fei
Cao, Li
Chen, Yu
Xia, Bing
Bi, Qin
Wang, Ya-Ping
author_sort Xu, Ji-Feng
collection PubMed
description The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal miRNAs isolated from human bone marrow-derived mesenchymal stem cells (BMSCs) culture were profiled using miRNA arrays containing probes for 894 human matured miRNAs. Seventy-nine miRNAs (∼8.84%) could be detected in exosomes isolated from BMSC culture supernatants when normalized to endogenous control genes RNU44. Among them, nine exosomal miRNAs were up regulated and 4 miRNAs were under regulated significantly (Relative fold>2, p<0.05) when compared with the values at 0 day with maximum changes at 1 to 7 days. Five miRNAs (miR-199b, miR-218, miR-148a, miR-135b, and miR-221) were further validated and differentially expressed in the individual exosomal samples from hBMSCs cultured at different time points. Bioinformatic analysis by DIANA-mirPath demonstrated that RNA degradation, mRNA surveillance pathway, Wnt signaling pathway, RNA transport were the most prominent pathways enriched in quantiles with differential exosomal miRNA patterns related to osteogenic differentiation. These data demonstrated exosomal miRNA is a regulator of osteoblast differentiation.
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spelling pubmed-42637342014-12-19 Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Xu, Ji-Feng Yang, Guang-hai Pan, Xiao-Hong Zhang, Shui-Jun Zhao, Chen Qiu, Bin-Song Gu, Hai-Feng Hong, Jian-Fei Cao, Li Chen, Yu Xia, Bing Bi, Qin Wang, Ya-Ping PLoS One Research Article The physiological role of microRNAs (miRNAs) in osteoblast differentiation remains elusive. Exosomal miRNAs isolated from human bone marrow-derived mesenchymal stem cells (BMSCs) culture were profiled using miRNA arrays containing probes for 894 human matured miRNAs. Seventy-nine miRNAs (∼8.84%) could be detected in exosomes isolated from BMSC culture supernatants when normalized to endogenous control genes RNU44. Among them, nine exosomal miRNAs were up regulated and 4 miRNAs were under regulated significantly (Relative fold>2, p<0.05) when compared with the values at 0 day with maximum changes at 1 to 7 days. Five miRNAs (miR-199b, miR-218, miR-148a, miR-135b, and miR-221) were further validated and differentially expressed in the individual exosomal samples from hBMSCs cultured at different time points. Bioinformatic analysis by DIANA-mirPath demonstrated that RNA degradation, mRNA surveillance pathway, Wnt signaling pathway, RNA transport were the most prominent pathways enriched in quantiles with differential exosomal miRNA patterns related to osteogenic differentiation. These data demonstrated exosomal miRNA is a regulator of osteoblast differentiation. Public Library of Science 2014-12-11 /pmc/articles/PMC4263734/ /pubmed/25503309 http://dx.doi.org/10.1371/journal.pone.0114627 Text en © 2014 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xu, Ji-Feng
Yang, Guang-hai
Pan, Xiao-Hong
Zhang, Shui-Jun
Zhao, Chen
Qiu, Bin-Song
Gu, Hai-Feng
Hong, Jian-Fei
Cao, Li
Chen, Yu
Xia, Bing
Bi, Qin
Wang, Ya-Ping
Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_full Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_fullStr Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_full_unstemmed Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_short Altered MicroRNA Expression Profile in Exosomes during Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells
title_sort altered microrna expression profile in exosomes during osteogenic differentiation of human bone marrow-derived mesenchymal stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263734/
https://www.ncbi.nlm.nih.gov/pubmed/25503309
http://dx.doi.org/10.1371/journal.pone.0114627
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