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Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii

Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encodin...

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Autores principales: Yan, Fang, Luo, Shaojie, Jiao, Yu, Deng, Yuewen, Du, Xiaodong, Huang, Ronglian, Wang, Qingheng, Chen, Weiyao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264221/
https://www.ncbi.nlm.nih.gov/pubmed/25407527
http://dx.doi.org/10.3390/ijms151121215
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author Yan, Fang
Luo, Shaojie
Jiao, Yu
Deng, Yuewen
Du, Xiaodong
Huang, Ronglian
Wang, Qingheng
Chen, Weiyao
author_facet Yan, Fang
Luo, Shaojie
Jiao, Yu
Deng, Yuewen
Du, Xiaodong
Huang, Ronglian
Wang, Qingheng
Chen, Weiyao
author_sort Yan, Fang
collection PubMed
description Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encoding a 429 amino acid polypeptide and a 3'-UTR of 1388 bp. The deduced protein sequence of Pm-BMP7 contained a signal peptide, a pro-domain and a mature peptide. The mature peptide consisted of 135 amino acids and included a transforming growth factor β family domain with six shared cysteine residues. The protein sequence of Pm-BMP7 showed 66% identity with that from Crassostrea gigas. Two unigenes encoding Pm-BMPRI (Pm-BMP receptor I) and Pm-BMPRII were obtained from the transcriptome database of P. martensii. Tissue expression analysis demonstrated Pm-BMP7 and Pm-BMPRI were highly expressed in the mantle (shell formation related-tissue), while Pm-BMPRII was highly expressed in the foot. After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue). The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group. These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell.
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spelling pubmed-42642212014-12-12 Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii Yan, Fang Luo, Shaojie Jiao, Yu Deng, Yuewen Du, Xiaodong Huang, Ronglian Wang, Qingheng Chen, Weiyao Int J Mol Sci Article Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encoding a 429 amino acid polypeptide and a 3'-UTR of 1388 bp. The deduced protein sequence of Pm-BMP7 contained a signal peptide, a pro-domain and a mature peptide. The mature peptide consisted of 135 amino acids and included a transforming growth factor β family domain with six shared cysteine residues. The protein sequence of Pm-BMP7 showed 66% identity with that from Crassostrea gigas. Two unigenes encoding Pm-BMPRI (Pm-BMP receptor I) and Pm-BMPRII were obtained from the transcriptome database of P. martensii. Tissue expression analysis demonstrated Pm-BMP7 and Pm-BMPRI were highly expressed in the mantle (shell formation related-tissue), while Pm-BMPRII was highly expressed in the foot. After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue). The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group. These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell. MDPI 2014-11-17 /pmc/articles/PMC4264221/ /pubmed/25407527 http://dx.doi.org/10.3390/ijms151121215 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Fang
Luo, Shaojie
Jiao, Yu
Deng, Yuewen
Du, Xiaodong
Huang, Ronglian
Wang, Qingheng
Chen, Weiyao
Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title_full Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title_fullStr Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title_full_unstemmed Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title_short Molecular Characterization of the BMP7 Gene and Its Potential Role in Shell Formation in Pinctada martensii
title_sort molecular characterization of the bmp7 gene and its potential role in shell formation in pinctada martensii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264221/
https://www.ncbi.nlm.nih.gov/pubmed/25407527
http://dx.doi.org/10.3390/ijms151121215
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