Cargando…

Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy

The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined b...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Kai, Guan, Shi-He, Zhang, Hao, Pan, Ying, Wu, Yuan-Yuan, Wang, Ai-Hua, Sun, Bei-Bei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264225/
https://www.ncbi.nlm.nih.gov/pubmed/25407531
http://dx.doi.org/10.3390/ijms151121286
_version_ 1782348696452595712
author Yang, Kai
Guan, Shi-He
Zhang, Hao
Pan, Ying
Wu, Yuan-Yuan
Wang, Ai-Hua
Sun, Bei-Bei
author_facet Yang, Kai
Guan, Shi-He
Zhang, Hao
Pan, Ying
Wu, Yuan-Yuan
Wang, Ai-Hua
Sun, Bei-Bei
author_sort Yang, Kai
collection PubMed
description The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined by enzyme linked immunosorbent assay (ELISA), and fluorescence-based quantitative real-time PCR (RT-qPCR) was used to measure IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with CHB. IL-8 protein expression was detected in liver biopsy tissues by immunohistochemistry. In addition, the differences in serum IL-8 and PBMCs mRNA levels were also observed in patients with different anti-viral responses to IFN-α. Compared to normal controls, serum IL-8 protein and mRNA levels were increased in CHB patients, IL-8 levels were positively correlated with the severity of liver inflammation/fibrosis. Moreover, serum IL-8 protein and mRNA levels were positively correlated with serum alanine aminotransferase (ALT) level and negatively correlated with serum prealbumin (PA) level. IL-8 expression was mainly located in portal area of liver tissues and was increased with the severity of liver inflammation and fibrosis stage. The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment. It is suggested that IL-8 might play important roles in the pathogenesis of CHB. Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment.
format Online
Article
Text
id pubmed-4264225
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42642252014-12-12 Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy Yang, Kai Guan, Shi-He Zhang, Hao Pan, Ying Wu, Yuan-Yuan Wang, Ai-Hua Sun, Bei-Bei Int J Mol Sci Article The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined by enzyme linked immunosorbent assay (ELISA), and fluorescence-based quantitative real-time PCR (RT-qPCR) was used to measure IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with CHB. IL-8 protein expression was detected in liver biopsy tissues by immunohistochemistry. In addition, the differences in serum IL-8 and PBMCs mRNA levels were also observed in patients with different anti-viral responses to IFN-α. Compared to normal controls, serum IL-8 protein and mRNA levels were increased in CHB patients, IL-8 levels were positively correlated with the severity of liver inflammation/fibrosis. Moreover, serum IL-8 protein and mRNA levels were positively correlated with serum alanine aminotransferase (ALT) level and negatively correlated with serum prealbumin (PA) level. IL-8 expression was mainly located in portal area of liver tissues and was increased with the severity of liver inflammation and fibrosis stage. The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment. It is suggested that IL-8 might play important roles in the pathogenesis of CHB. Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment. MDPI 2014-11-17 /pmc/articles/PMC4264225/ /pubmed/25407531 http://dx.doi.org/10.3390/ijms151121286 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Kai
Guan, Shi-He
Zhang, Hao
Pan, Ying
Wu, Yuan-Yuan
Wang, Ai-Hua
Sun, Bei-Bei
Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title_full Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title_fullStr Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title_full_unstemmed Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title_short Enhanced Levels of Interleukin-8 Are Associated with Hepatitis B Virus Infection and Resistance to Interferon-Alpha Therapy
title_sort enhanced levels of interleukin-8 are associated with hepatitis b virus infection and resistance to interferon-alpha therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264225/
https://www.ncbi.nlm.nih.gov/pubmed/25407531
http://dx.doi.org/10.3390/ijms151121286
work_keys_str_mv AT yangkai enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT guanshihe enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT zhanghao enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT panying enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT wuyuanyuan enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT wangaihua enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy
AT sunbeibei enhancedlevelsofinterleukin8areassociatedwithhepatitisbvirusinfectionandresistancetointerferonalphatherapy