Cargando…

Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling

The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans,...

Descripción completa

Detalles Bibliográficos
Autores principales: Kopp, Christina, Hosseini, Afshin, Singh, Shiva P., Regenhard, Petra, Khalilvandi-Behroozyar, Hamed, Sauerwein, Helga, Mielenz, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264232/
https://www.ncbi.nlm.nih.gov/pubmed/25411802
http://dx.doi.org/10.3390/ijms151121401
_version_ 1782348698139754496
author Kopp, Christina
Hosseini, Afshin
Singh, Shiva P.
Regenhard, Petra
Khalilvandi-Behroozyar, Hamed
Sauerwein, Helga
Mielenz, Manfred
author_facet Kopp, Christina
Hosseini, Afshin
Singh, Shiva P.
Regenhard, Petra
Khalilvandi-Behroozyar, Hamed
Sauerwein, Helga
Mielenz, Manfred
author_sort Kopp, Christina
collection PubMed
description The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.
format Online
Article
Text
id pubmed-4264232
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-42642322014-12-12 Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling Kopp, Christina Hosseini, Afshin Singh, Shiva P. Regenhard, Petra Khalilvandi-Behroozyar, Hamed Sauerwein, Helga Mielenz, Manfred Int J Mol Sci Article The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows. MDPI 2014-11-18 /pmc/articles/PMC4264232/ /pubmed/25411802 http://dx.doi.org/10.3390/ijms151121401 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kopp, Christina
Hosseini, Afshin
Singh, Shiva P.
Regenhard, Petra
Khalilvandi-Behroozyar, Hamed
Sauerwein, Helga
Mielenz, Manfred
Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title_full Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title_fullStr Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title_full_unstemmed Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title_short Nicotinic Acid Increases Adiponectin Secretion from Differentiated Bovine Preadipocytes through G-Protein Coupled Receptor Signaling
title_sort nicotinic acid increases adiponectin secretion from differentiated bovine preadipocytes through g-protein coupled receptor signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264232/
https://www.ncbi.nlm.nih.gov/pubmed/25411802
http://dx.doi.org/10.3390/ijms151121401
work_keys_str_mv AT koppchristina nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT hosseiniafshin nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT singhshivap nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT regenhardpetra nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT khalilvandibehroozyarhamed nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT sauerweinhelga nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling
AT mielenzmanfred nicotinicacidincreasesadiponectinsecretionfromdifferentiatedbovinepreadipocytesthroughgproteincoupledreceptorsignaling