Cargando…

Assessing the Accuracy of Quantitative Molecular Microbial Profiling

The application of high-throughput sequencing in profiling microbial communities is providing an unprecedented ability to investigate microbiomes. Such studies typically apply one of two methods: amplicon sequencing using PCR to target a conserved orthologous sequence (typically the 16S ribosomal RN...

Descripción completa

Detalles Bibliográficos
Autores principales:	O’Sullivan, Denise M., Laver, Thomas, Temisak, Sasithon, Redshaw, Nicholas, Harris, Kathryn A., Foy, Carole A., Studholme, David J., Huggett, Jim F.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264237/ https://www.ncbi.nlm.nih.gov/pubmed/25421243 http://dx.doi.org/10.3390/ijms151121476

Ejemplares similares

Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis
por: Redshaw, Nicholas, et al.
Publicado: (2014)

Considerations for accurate gene expression measurement by reverse transcription quantitative PCR when analysing clinical samples
por: Sanders, Rebecca, et al.
Publicado: (2014)

An inter-laboratory study to investigate the impact of the bioinformatics component on microbiome analysis using mock communities
por: O’Sullivan, Denise M., et al.
Publicado: (2021)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples
por: Whale, Alexandra S., et al.
Publicado: (2013)

The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis
por: Devonshire, Alison S., et al.
Publicado: (2016)

Evaluation of Digital PCR for Absolute RNA Quantification
por: Sanders, Rebecca, et al.
Publicado: (2013)

Validation of droplet digital PCR for cytokeratin 19 mRNA detection in canine peripheral blood and mammary gland
por: Tanvetthayanont, Potsawat, et al.
Publicado: (2022)

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples
por: Whale, Alexandra S., et al.
Publicado: (2016)

Single base mutations in the nucleocapsid gene of SARS-CoV-2 affects amplification efficiency of sequence variants and may lead to assay failure
por: Storey, Nathaniel, et al.
Publicado: (2021)

A novel approach for evaluating the performance of real time quantitative loop-mediated isothermal amplification-based methods
por: Nixon, Gavin J., et al.
Publicado: (2014)

Monkeypox: another test for PCR
por: Huggett, Jim F, et al.
Publicado: (2022)

Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes
por: Brown, Julianne R., et al.
Publicado: (2021)

Inter-laboratory assessment of different digital PCR platforms for quantification of human cytomegalovirus DNA
por: Pavšič, Jernej, et al.
Publicado: (2017)

Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification
por: Devonshire, Alison S., et al.
Publicado: (2014)

Applicability of Control Materials To Support Gene Promoter Characterization and Expression in Engineered Cells Using Digital PCR
por: Fernandez-Gonzalez, Ana, et al.
Publicado: (2022)

Standardisation of data from real-time quantitative PCR methods – evaluation of outliers and comparison of calibration curves
por: Burns, Malcolm J, et al.
Publicado: (2005)

Evaluation of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumannii in Comparison with Orthogonal Methods
por: Busby, Eloise J., et al.
Publicado: (2023)

Identification of reference genes for circulating long noncoding RNA analysis in serum of cervical cancer patients
por: Iempridee, Tawin, et al.
Publicado: (2018)

Rapid quantitative profiling of complex microbial populations
por: Palmer, Chana, et al.
Publicado: (2006)

Immersion Vaccination by a Biomimetic-Mucoadhesive Nanovaccine Induces Humoral Immune Response of Red Tilapia (Oreochromis sp.) against Flavobacterium columnare Challenge
por: Kitiyodom, Sirikorn, et al.
Publicado: (2021)

Thermoresponsive Bacteriophage Nanocarrier as a Gene Delivery Vector Targeted to the Gastrointestinal Tract
por: Namdee, Katawut, et al.
Publicado: (2018)

Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR
por: Busby, Eloise, et al.
Publicado: (2017)

Making standards for quantitative real-time pneumococcal PCR
por: Morpeth, Susan C., et al.
Publicado: (2014)

Systematic review with meta-analysis of diagnostic test accuracy for COVID-19 by mass spectrometry
por: Spick, Matt, et al.
Publicado: (2022)

Reproducibility, stability, and accuracy of microbial profiles by fecal sample collection method in three distinct populations
por: Byrd, Doratha A., et al.
Publicado: (2019)

Assessing the performance of the Oxford Nanopore Technologies MinION
por: Laver, T., et al.
Publicado: (2015)

Mothers’ experience of maternity and neonatal care when babies die: A quantitative study
por: Redshaw, Maggie, et al.
Publicado: (2018)

Specialist antenatal clinics for women at high risk of preterm birth: a systematic review of qualitative and quantitative research
por: Malouf, Reem, et al.
Publicado: (2017)

Microbial Succession and Flavor Production in the Fermented Dairy Beverage Kefir
por: Walsh, Aaron M., et al.
Publicado: (2016)

The variability and reproducibility of whole genome sequencing technology for detecting resistance to anti-tuberculous drugs
por: Phelan, Jody, et al.
Publicado: (2016)

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments
por: Bustin, Stephen A, et al.
Publicado: (2010)

Reproducibility of biomedical research – The importance of editorial vigilance
por: Bustin, Stephen A., et al.
Publicado: (2017)

Quantitative analysis of human endogenous retrovirus-K transcripts in postmortem premotor cortex fails to confirm elevated expression of HERV-K RNA in amyotrophic lateral sclerosis
por: Garson, Jeremy A., et al.
Publicado: (2019)

por: Taly, Valerie, et al.
Publicado: (2016)

qPCR primer design revisited
por: Bustin, Stephen, et al.
Publicado: (2017)

International interlaboratory study comparing single organism 16S rRNA gene sequencing data: Beyond consensus sequence comparisons
por: Olson, Nathan D., et al.
Publicado: (2015)

Integrating informatics tools and portable sequencing technology for rapid detection of resistance to anti-tuberculous drugs
por: Phelan, Jody E., et al.
Publicado: (2019)

Quantitative accuracy of (177)Lu SPECT imaging for molecular radiotherapy
por: Mezzenga, Emilio, et al.
Publicado: (2017)

Correction for Walsh et al., Microbial Succession and Flavor Production in the Fermented Dairy Beverage Kefir
por: Walsh, Aaron M., et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_joe29sch8l6pn6kp4makdk1alg