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Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors
AIMS: Nuclear size increases in malignant tumors and reflects DNA content, ploidy and proliferation index. Present study investigated if the nuclear morphometry could differentiate histomorphologically similar paediatric malignant small round cell tumors on hematoxylin and eosin stained sections for...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264272/ https://www.ncbi.nlm.nih.gov/pubmed/25538403 http://dx.doi.org/10.4103/0971-5851.144987 |
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author | Bansal, Cherry Gupta, Archana Kumar, Archana Srivastava, Anand |
author_facet | Bansal, Cherry Gupta, Archana Kumar, Archana Srivastava, Anand |
author_sort | Bansal, Cherry |
collection | PubMed |
description | AIMS: Nuclear size increases in malignant tumors and reflects DNA content, ploidy and proliferation index. Present study investigated if the nuclear morphometry could differentiate histomorphologically similar paediatric malignant small round cell tumors on hematoxylin and eosin stained sections for diagnostics in a resource poor setting. SETTINGS AND DESIGN: All the consecutive malignant pediatric tumors received in Pathology Department from other faculties of King George's Medical University and also those referred directly to Pathology Department from other hospitals of city/other cities during 3 years period were recorded. MATERIALS AND METHODS: Morphometric analysis was done in 22 confirmed (by higher ancillary techniques) but histomorphologically difficult to differentiate round cell tumors. All sections were analyzed by cell images from six different areas, using Leica Q win 500 images software. RESULTS: Nuclear measurements were obtained for retinoblastoma (RB) (nine cases), neuroblastoma (five cases), Wilms tumor (WT) (three cases), rhabdomyosarcoma (three cases), malignant hemangiopericytoma (one case) and non-Hodgkin lymphoma (one case). Among the RBs, maximum mean nuclear area percent (24.93) was seen in a case with nerve involvement and metastasis, followed by cases with only nerve involvement (21.60) and smallest area (16.57) was in non-nerve involving, nonmetastatic cases. All five cases of neuroblastoma had almost similar mean nuclear area percent (18.05-18.29). WT case with metastasis had higher nuclear area (21.25) than nonmetastatic (19.47). Amongst all the tumors, minimum value (14.93) was seen in malignant hemangiopericytoma. CONCLUSION: Morphometric evaluation in paediatric malignant round cell tumors have generated useful data, and needs further multicentric confirmation for implementation. |
format | Online Article Text |
id | pubmed-4264272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42642722014-12-23 Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors Bansal, Cherry Gupta, Archana Kumar, Archana Srivastava, Anand Indian J Med Paediatr Oncol Original Article AIMS: Nuclear size increases in malignant tumors and reflects DNA content, ploidy and proliferation index. Present study investigated if the nuclear morphometry could differentiate histomorphologically similar paediatric malignant small round cell tumors on hematoxylin and eosin stained sections for diagnostics in a resource poor setting. SETTINGS AND DESIGN: All the consecutive malignant pediatric tumors received in Pathology Department from other faculties of King George's Medical University and also those referred directly to Pathology Department from other hospitals of city/other cities during 3 years period were recorded. MATERIALS AND METHODS: Morphometric analysis was done in 22 confirmed (by higher ancillary techniques) but histomorphologically difficult to differentiate round cell tumors. All sections were analyzed by cell images from six different areas, using Leica Q win 500 images software. RESULTS: Nuclear measurements were obtained for retinoblastoma (RB) (nine cases), neuroblastoma (five cases), Wilms tumor (WT) (three cases), rhabdomyosarcoma (three cases), malignant hemangiopericytoma (one case) and non-Hodgkin lymphoma (one case). Among the RBs, maximum mean nuclear area percent (24.93) was seen in a case with nerve involvement and metastasis, followed by cases with only nerve involvement (21.60) and smallest area (16.57) was in non-nerve involving, nonmetastatic cases. All five cases of neuroblastoma had almost similar mean nuclear area percent (18.05-18.29). WT case with metastasis had higher nuclear area (21.25) than nonmetastatic (19.47). Amongst all the tumors, minimum value (14.93) was seen in malignant hemangiopericytoma. CONCLUSION: Morphometric evaluation in paediatric malignant round cell tumors have generated useful data, and needs further multicentric confirmation for implementation. Medknow Publications & Media Pvt Ltd 2014 /pmc/articles/PMC4264272/ /pubmed/25538403 http://dx.doi.org/10.4103/0971-5851.144987 Text en Copyright: © Indian Journal of Medical and Paediatric Oncology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bansal, Cherry Gupta, Archana Kumar, Archana Srivastava, Anand Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title | Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title_full | Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title_fullStr | Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title_full_unstemmed | Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title_short | Morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
title_sort | morphometric evaluation and clinical correlations in pediatric malignant small round cell tumors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264272/ https://www.ncbi.nlm.nih.gov/pubmed/25538403 http://dx.doi.org/10.4103/0971-5851.144987 |
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