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Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis

We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding th...

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Detalles Bibliográficos
Autores principales: Li, Yunzi, Burke, David T., Kopelman, Raoul, Burns, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264372/
https://www.ncbi.nlm.nih.gov/pubmed/25587411
http://dx.doi.org/10.3390/bios4010076
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author Li, Yunzi
Burke, David T.
Kopelman, Raoul
Burns, Mark A.
author_facet Li, Yunzi
Burke, David T.
Kopelman, Raoul
Burns, Mark A.
author_sort Li, Yunzi
collection PubMed
description We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.
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spelling pubmed-42643722015-01-13 Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis Li, Yunzi Burke, David T. Kopelman, Raoul Burns, Mark A. Biosensors (Basel) Article We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes. MDPI 2014-03-21 /pmc/articles/PMC4264372/ /pubmed/25587411 http://dx.doi.org/10.3390/bios4010076 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Li, Yunzi
Burke, David T.
Kopelman, Raoul
Burns, Mark A.
Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title_full Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title_fullStr Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title_full_unstemmed Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title_short Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis
title_sort asynchronous magnetic bead rotation (ambr) microviscometer for label-free dna analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264372/
https://www.ncbi.nlm.nih.gov/pubmed/25587411
http://dx.doi.org/10.3390/bios4010076
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