A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety

Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety dec...

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Detalles Bibliográficos
Autores principales: Elmlund, Louise, Söderberg, Pernilla, Suriyanarayanan, Subramanian, Nicholls, Ian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264375/
https://www.ncbi.nlm.nih.gov/pubmed/25587414
http://dx.doi.org/10.3390/bios4020137
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author Elmlund, Louise
Söderberg, Pernilla
Suriyanarayanan, Subramanian
Nicholls, Ian A.
author_facet Elmlund, Louise
Söderberg, Pernilla
Suriyanarayanan, Subramanian
Nicholls, Ian A.
author_sort Elmlund, Louise
collection PubMed
description Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (K(D) of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions.
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spelling pubmed-42643752015-01-13 A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety Elmlund, Louise Söderberg, Pernilla Suriyanarayanan, Subramanian Nicholls, Ian A. Biosensors (Basel) Article Phage display screening of a surface-immobilized adenine derivative led to the identification of a heptameric peptide with selectivity for adenine as demonstrated through quartz crystal microbalance (QCM) studies. The peptide demonstrated a concentration dependent affinity for an adeninyl moiety decorated surface (K(D) of 968 ± 53.3 μM), which highlights the power of piezoelectric sensing in the study of weak interactions. MDPI 2014-04-29 /pmc/articles/PMC4264375/ /pubmed/25587414 http://dx.doi.org/10.3390/bios4020137 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Elmlund, Louise
Söderberg, Pernilla
Suriyanarayanan, Subramanian
Nicholls, Ian A.
A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title_full A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title_fullStr A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title_full_unstemmed A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title_short A Phage Display Screening Derived Peptide with Affinity for the Adeninyl Moiety
title_sort phage display screening derived peptide with affinity for the adeninyl moiety
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264375/
https://www.ncbi.nlm.nih.gov/pubmed/25587414
http://dx.doi.org/10.3390/bios4020137
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