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Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP

Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. T...

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Autores principales: Phan, Nam V. H., Sussitz, Hermann F., Lieberzeit, Peter A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264377/
https://www.ncbi.nlm.nih.gov/pubmed/25587416
http://dx.doi.org/10.3390/bios4020161
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author Phan, Nam V. H.
Sussitz, Hermann F.
Lieberzeit, Peter A.
author_facet Phan, Nam V. H.
Sussitz, Hermann F.
Lieberzeit, Peter A.
author_sort Phan, Nam V. H.
collection PubMed
description Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance—QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.
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spelling pubmed-42643772015-01-13 Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP Phan, Nam V. H. Sussitz, Hermann F. Lieberzeit, Peter A. Biosensors (Basel) Article Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance—QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity. MDPI 2014-06-16 /pmc/articles/PMC4264377/ /pubmed/25587416 http://dx.doi.org/10.3390/bios4020161 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Phan, Nam V. H.
Sussitz, Hermann F.
Lieberzeit, Peter A.
Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title_full Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title_fullStr Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title_full_unstemmed Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title_short Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP
title_sort polymerization parameters influencing the qcm response characteristics of bsa mip
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264377/
https://www.ncbi.nlm.nih.gov/pubmed/25587416
http://dx.doi.org/10.3390/bios4020161
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