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HmsB enhances biofilm formation in Yersinia pestis

The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide. Yersinia pestis expresses the two sole diguanylate cyclases HmsT and HmsD and the sole phosphodiesterase HmsP, which are specific for biosynthesis and degradation, respectively, of 3′,5′-cyclic...

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Autores principales: Fang, Nan, Qu, Shi, Yang, Huiying, Fang, Haihong, Liu, Lei, Zhang, Yiquan, Wang, Li, Han, Yanping, Zhou, Dongsheng, Yang, Ruifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264472/
https://www.ncbi.nlm.nih.gov/pubmed/25566205
http://dx.doi.org/10.3389/fmicb.2014.00685
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author Fang, Nan
Qu, Shi
Yang, Huiying
Fang, Haihong
Liu, Lei
Zhang, Yiquan
Wang, Li
Han, Yanping
Zhou, Dongsheng
Yang, Ruifu
author_facet Fang, Nan
Qu, Shi
Yang, Huiying
Fang, Haihong
Liu, Lei
Zhang, Yiquan
Wang, Li
Han, Yanping
Zhou, Dongsheng
Yang, Ruifu
author_sort Fang, Nan
collection PubMed
description The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide. Yersinia pestis expresses the two sole diguanylate cyclases HmsT and HmsD and the sole phosphodiesterase HmsP, which are specific for biosynthesis and degradation, respectively, of 3′,5′-cyclic diguanosine monophosphate (c-di-GMP), a second messenger promoting exopolysaccharide production. In this work, the phenotypic assays indicates that Y. pestis sRNA HmsB enhances the production of c-di-GMP, exopolysaccharide, and biofilm. Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP. HmsB most likely acts as a major activator of biofilm formation in Y. pestis. This is the first report of regulation of Yersinia biofilm formation by a sRNA. Data presented here will promote us to gain a deeper understanding of the complex regulatory circuits controlling Yersinia biofilm formation.
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spelling pubmed-42644722015-01-06 HmsB enhances biofilm formation in Yersinia pestis Fang, Nan Qu, Shi Yang, Huiying Fang, Haihong Liu, Lei Zhang, Yiquan Wang, Li Han, Yanping Zhou, Dongsheng Yang, Ruifu Front Microbiol Microbiology The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide. Yersinia pestis expresses the two sole diguanylate cyclases HmsT and HmsD and the sole phosphodiesterase HmsP, which are specific for biosynthesis and degradation, respectively, of 3′,5′-cyclic diguanosine monophosphate (c-di-GMP), a second messenger promoting exopolysaccharide production. In this work, the phenotypic assays indicates that Y. pestis sRNA HmsB enhances the production of c-di-GMP, exopolysaccharide, and biofilm. Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP. HmsB most likely acts as a major activator of biofilm formation in Y. pestis. This is the first report of regulation of Yersinia biofilm formation by a sRNA. Data presented here will promote us to gain a deeper understanding of the complex regulatory circuits controlling Yersinia biofilm formation. Frontiers Media S.A. 2014-12-12 /pmc/articles/PMC4264472/ /pubmed/25566205 http://dx.doi.org/10.3389/fmicb.2014.00685 Text en Copyright © 2014 Fang, Qu, Yang, Fang, Liu, Zhang, Wang, Han, Zhou and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Fang, Nan
Qu, Shi
Yang, Huiying
Fang, Haihong
Liu, Lei
Zhang, Yiquan
Wang, Li
Han, Yanping
Zhou, Dongsheng
Yang, Ruifu
HmsB enhances biofilm formation in Yersinia pestis
title HmsB enhances biofilm formation in Yersinia pestis
title_full HmsB enhances biofilm formation in Yersinia pestis
title_fullStr HmsB enhances biofilm formation in Yersinia pestis
title_full_unstemmed HmsB enhances biofilm formation in Yersinia pestis
title_short HmsB enhances biofilm formation in Yersinia pestis
title_sort hmsb enhances biofilm formation in yersinia pestis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264472/
https://www.ncbi.nlm.nih.gov/pubmed/25566205
http://dx.doi.org/10.3389/fmicb.2014.00685
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