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PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana

The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of thre...

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Autores principales: Humphrey, Tania V., Haasen, Katrina E., Aldea-Brydges, May Grace, Sun, He, Zayed, Yara, Indriolo, Emily, Goring, Daphne R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265151/
https://www.ncbi.nlm.nih.gov/pubmed/25262228
http://dx.doi.org/10.1093/jxb/eru390
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author Humphrey, Tania V.
Haasen, Katrina E.
Aldea-Brydges, May Grace
Sun, He
Zayed, Yara
Indriolo, Emily
Goring, Daphne R.
author_facet Humphrey, Tania V.
Haasen, Katrina E.
Aldea-Brydges, May Grace
Sun, He
Zayed, Yara
Indriolo, Emily
Goring, Daphne R.
author_sort Humphrey, Tania V.
collection PubMed
description The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of three Arabidopsis predicted PERK genes, PERK8, -9, and -10, for which no functions were known. Yeast two-hybrid interaction studies were conducted with the PERK8,- 9, and -10 cytosolic kinase domains, and two members of the Arabidopsis AGC VIII kinase family were identified as interacting proteins: AGC1-9 and the closely related kinesin-like calmodulin-binding protein (KCBP)-interacting protein kinase (KIPK). As KIPK has been identified previously as an interactor of KCBP, these interactions were also examined further and confirmed in this study. Finally, T-DNA mutants for each gene were screened for altered phenotypes under different conditions, and from these screens, a role for the PERK, KIPK, and KCBP genes in negatively regulating root growth was uncovered.
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spelling pubmed-42651512015-03-24 PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana Humphrey, Tania V. Haasen, Katrina E. Aldea-Brydges, May Grace Sun, He Zayed, Yara Indriolo, Emily Goring, Daphne R. J Exp Bot Research Paper The Arabidopsis proline-rich, extensin-like receptor-like kinases (PERKs) are a small group of receptor-like kinases that are thought to act as sensors at the cell wall through their predicted proline-rich extracellular domains. In this study, we focused on the characterization of a subclade of three Arabidopsis predicted PERK genes, PERK8, -9, and -10, for which no functions were known. Yeast two-hybrid interaction studies were conducted with the PERK8,- 9, and -10 cytosolic kinase domains, and two members of the Arabidopsis AGC VIII kinase family were identified as interacting proteins: AGC1-9 and the closely related kinesin-like calmodulin-binding protein (KCBP)-interacting protein kinase (KIPK). As KIPK has been identified previously as an interactor of KCBP, these interactions were also examined further and confirmed in this study. Finally, T-DNA mutants for each gene were screened for altered phenotypes under different conditions, and from these screens, a role for the PERK, KIPK, and KCBP genes in negatively regulating root growth was uncovered. Oxford University Press 2015-01 2014-09-26 /pmc/articles/PMC4265151/ /pubmed/25262228 http://dx.doi.org/10.1093/jxb/eru390 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Humphrey, Tania V.
Haasen, Katrina E.
Aldea-Brydges, May Grace
Sun, He
Zayed, Yara
Indriolo, Emily
Goring, Daphne R.
PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title_full PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title_fullStr PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title_full_unstemmed PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title_short PERK–KIPK–KCBP signalling negatively regulates root growth in Arabidopsis thaliana
title_sort perk–kipk–kcbp signalling negatively regulates root growth in arabidopsis thaliana
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265151/
https://www.ncbi.nlm.nih.gov/pubmed/25262228
http://dx.doi.org/10.1093/jxb/eru390
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