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Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures
Although rising ocean temperatures threaten scleractinian corals and the reefs they construct, certain reef corals can acclimate to elevated temperatures to which they are rarely exposed in situ. Specimens of the model Indo-Pacific reef coral Pocillopora damicornis collected from upwelling reefs of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BlackWell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265203/ https://www.ncbi.nlm.nih.gov/pubmed/25354956 http://dx.doi.org/10.1111/mec.12982 |
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author | Mayfield, Anderson B Wang, Yu-Bin Chen, Chii-Shiarng Lin, Chung-Yen Chen, Shu-Hwa |
author_facet | Mayfield, Anderson B Wang, Yu-Bin Chen, Chii-Shiarng Lin, Chung-Yen Chen, Shu-Hwa |
author_sort | Mayfield, Anderson B |
collection | PubMed |
description | Although rising ocean temperatures threaten scleractinian corals and the reefs they construct, certain reef corals can acclimate to elevated temperatures to which they are rarely exposed in situ. Specimens of the model Indo-Pacific reef coral Pocillopora damicornis collected from upwelling reefs of Southern Taiwan were previously found to have survived a 36-week exposure to 30°C, a temperature they encounter infrequently and one that can elicit the breakdown of the coral–dinoflagellate (genus Symbiodinium) endosymbiosis in many corals of the Pacific Ocean. To gain insight into the subcellular pathways utilized by both the coral hosts and their mutualistic Symbiodinium populations to acclimate to this temperature, mRNAs from both control (27°C) and high (30°C)-temperature samples were sequenced on an Illumina platform and assembled into a 236 435-contig transcriptome. These P. damicornis specimens were found to be ∼60% anthozoan and 40% microbe (Symbiodinium, other eukaryotic microbes, and bacteria), from an mRNA-perspective. Furthermore, a significantly higher proportion of genes from the Symbiodinium compartment were differentially expressed after two weeks of exposure. Specifically, at elevated temperatures, Symbiodinium populations residing within the coral gastrodermal tissues were more likely to up-regulate the expression of genes encoding proteins involved in metabolism than their coral hosts. Collectively, these transcriptome-scale data suggest that the two members of this endosymbiosis have distinct strategies for acclimating to elevated temperatures that are expected to characterize many of Earth's coral reefs in the coming decades. |
format | Online Article Text |
id | pubmed-4265203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BlackWell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-42652032014-12-19 Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures Mayfield, Anderson B Wang, Yu-Bin Chen, Chii-Shiarng Lin, Chung-Yen Chen, Shu-Hwa Mol Ecol Original Articles Although rising ocean temperatures threaten scleractinian corals and the reefs they construct, certain reef corals can acclimate to elevated temperatures to which they are rarely exposed in situ. Specimens of the model Indo-Pacific reef coral Pocillopora damicornis collected from upwelling reefs of Southern Taiwan were previously found to have survived a 36-week exposure to 30°C, a temperature they encounter infrequently and one that can elicit the breakdown of the coral–dinoflagellate (genus Symbiodinium) endosymbiosis in many corals of the Pacific Ocean. To gain insight into the subcellular pathways utilized by both the coral hosts and their mutualistic Symbiodinium populations to acclimate to this temperature, mRNAs from both control (27°C) and high (30°C)-temperature samples were sequenced on an Illumina platform and assembled into a 236 435-contig transcriptome. These P. damicornis specimens were found to be ∼60% anthozoan and 40% microbe (Symbiodinium, other eukaryotic microbes, and bacteria), from an mRNA-perspective. Furthermore, a significantly higher proportion of genes from the Symbiodinium compartment were differentially expressed after two weeks of exposure. Specifically, at elevated temperatures, Symbiodinium populations residing within the coral gastrodermal tissues were more likely to up-regulate the expression of genes encoding proteins involved in metabolism than their coral hosts. Collectively, these transcriptome-scale data suggest that the two members of this endosymbiosis have distinct strategies for acclimating to elevated temperatures that are expected to characterize many of Earth's coral reefs in the coming decades. BlackWell Publishing Ltd 2014-12 2014-11-27 /pmc/articles/PMC4265203/ /pubmed/25354956 http://dx.doi.org/10.1111/mec.12982 Text en © 2014 The Authors. Molecular Ecology Published by John Wiley & Sons Ltd. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Mayfield, Anderson B Wang, Yu-Bin Chen, Chii-Shiarng Lin, Chung-Yen Chen, Shu-Hwa Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title | Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title_full | Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title_fullStr | Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title_full_unstemmed | Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title_short | Compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
title_sort | compartment-specific transcriptomics in a reef-building coral exposed to elevated temperatures |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265203/ https://www.ncbi.nlm.nih.gov/pubmed/25354956 http://dx.doi.org/10.1111/mec.12982 |
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