Cargando…

Evidence of West Nile virus infection in Nepal

BACKGROUND: Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepa...

Descripción completa

Detalles Bibliográficos
Autores principales: Rutvisuttinunt, Wiriya, Chinnawirotpisan, Piyawan, Klungthong, Chonticha, Shrestha, Sanjaya Kumar, Thapa, Amod Bahadur, Pant, Arjun, Yingst, Samuel L, Yoon, In-Kyu, Fernandez, Stefan, Pavlin, Julie A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265323/
https://www.ncbi.nlm.nih.gov/pubmed/25427544
http://dx.doi.org/10.1186/s12879-014-0606-0
_version_ 1782348865074102272
author Rutvisuttinunt, Wiriya
Chinnawirotpisan, Piyawan
Klungthong, Chonticha
Shrestha, Sanjaya Kumar
Thapa, Amod Bahadur
Pant, Arjun
Yingst, Samuel L
Yoon, In-Kyu
Fernandez, Stefan
Pavlin, Julie A
author_facet Rutvisuttinunt, Wiriya
Chinnawirotpisan, Piyawan
Klungthong, Chonticha
Shrestha, Sanjaya Kumar
Thapa, Amod Bahadur
Pant, Arjun
Yingst, Samuel L
Yoon, In-Kyu
Fernandez, Stefan
Pavlin, Julie A
author_sort Rutvisuttinunt, Wiriya
collection PubMed
description BACKGROUND: Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepal. METHOD: Study methods included diagnostic testing and preliminary ELISA screening of acute and convalescent samples for diseases both known and unknown to be circulating in Nepal, including West Nile virus (WNV). The molecular assays including Polymerase Chain Reaction (PCR), Sanger sequencing and ultra deep sequencing on MiSeq Illumina Platform were conducted to further confirm the presence of WNV. RESULTS: The study enrolled 2,046 patients presenting undifferentiated febrile illness with unknown etiology. Sera from 14 out of 2,046 patients were tested positive for west nile virus (WNV) by nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Only two out of 14 cases were confirmed for the presence of WNV by sequencing and identified as WNV lineage 1 phylogentically. The two patients were adult males with fever and no neurological symptoms from Kathmandu and Bharatpur, Nepal. CONCLUSION: Two out of 2,046 serum samples contained fragments of WNV genome resembling WNV lineage 1, which is evidence of the continued spread of WNV which should be considered a possible illness cause in Nepal. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-014-0606-0) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4265323
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-42653232014-12-14 Evidence of West Nile virus infection in Nepal Rutvisuttinunt, Wiriya Chinnawirotpisan, Piyawan Klungthong, Chonticha Shrestha, Sanjaya Kumar Thapa, Amod Bahadur Pant, Arjun Yingst, Samuel L Yoon, In-Kyu Fernandez, Stefan Pavlin, Julie A BMC Infect Dis Research Article BACKGROUND: Acute febrile illness is common among those seeking medical care and is frequently treated empirically with the underlying illness remaining undiagnosed in resource-poor countries. A febrile illness study was conducted 2009-2010 to identify known and unknown pathogens circulating in Nepal. METHOD: Study methods included diagnostic testing and preliminary ELISA screening of acute and convalescent samples for diseases both known and unknown to be circulating in Nepal, including West Nile virus (WNV). The molecular assays including Polymerase Chain Reaction (PCR), Sanger sequencing and ultra deep sequencing on MiSeq Illumina Platform were conducted to further confirm the presence of WNV. RESULTS: The study enrolled 2,046 patients presenting undifferentiated febrile illness with unknown etiology. Sera from 14 out of 2,046 patients were tested positive for west nile virus (WNV) by nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR). Only two out of 14 cases were confirmed for the presence of WNV by sequencing and identified as WNV lineage 1 phylogentically. The two patients were adult males with fever and no neurological symptoms from Kathmandu and Bharatpur, Nepal. CONCLUSION: Two out of 2,046 serum samples contained fragments of WNV genome resembling WNV lineage 1, which is evidence of the continued spread of WNV which should be considered a possible illness cause in Nepal. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-014-0606-0) contains supplementary material, which is available to authorized users. BioMed Central 2014-11-27 /pmc/articles/PMC4265323/ /pubmed/25427544 http://dx.doi.org/10.1186/s12879-014-0606-0 Text en © Rutvisuttinunt et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Rutvisuttinunt, Wiriya
Chinnawirotpisan, Piyawan
Klungthong, Chonticha
Shrestha, Sanjaya Kumar
Thapa, Amod Bahadur
Pant, Arjun
Yingst, Samuel L
Yoon, In-Kyu
Fernandez, Stefan
Pavlin, Julie A
Evidence of West Nile virus infection in Nepal
title Evidence of West Nile virus infection in Nepal
title_full Evidence of West Nile virus infection in Nepal
title_fullStr Evidence of West Nile virus infection in Nepal
title_full_unstemmed Evidence of West Nile virus infection in Nepal
title_short Evidence of West Nile virus infection in Nepal
title_sort evidence of west nile virus infection in nepal
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265323/
https://www.ncbi.nlm.nih.gov/pubmed/25427544
http://dx.doi.org/10.1186/s12879-014-0606-0
work_keys_str_mv AT rutvisuttinuntwiriya evidenceofwestnilevirusinfectioninnepal
AT chinnawirotpisanpiyawan evidenceofwestnilevirusinfectioninnepal
AT klungthongchonticha evidenceofwestnilevirusinfectioninnepal
AT shresthasanjayakumar evidenceofwestnilevirusinfectioninnepal
AT thapaamodbahadur evidenceofwestnilevirusinfectioninnepal
AT pantarjun evidenceofwestnilevirusinfectioninnepal
AT yingstsamuell evidenceofwestnilevirusinfectioninnepal
AT yooninkyu evidenceofwestnilevirusinfectioninnepal
AT fernandezstefan evidenceofwestnilevirusinfectioninnepal
AT pavlinjuliea evidenceofwestnilevirusinfectioninnepal