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Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model

PURPOSE: To evaluate tumor-specific immunity and define the mechanisms involved in the cryoimmunologic response, we compared the tumor control efficacy and immunologic responses of cryoablation with those of surgical excision in a tumor rechallenge model. MATERIALS AND METHODS: Sixty BALB/c mice wit...

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Autores principales: Kim, Hyung Keun, Pyun, Jong Hyun, Cho, Seok, Kang, Sung Gu, Lee, Jeong Gu, Kim, Je Jong, Cheon, Jun, Park, Hong Seok, Kang, Seok Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Urological Association 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265719/
https://www.ncbi.nlm.nih.gov/pubmed/25512819
http://dx.doi.org/10.4111/kju.2014.55.12.834
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author Kim, Hyung Keun
Pyun, Jong Hyun
Cho, Seok
Kang, Sung Gu
Lee, Jeong Gu
Kim, Je Jong
Cheon, Jun
Park, Hong Seok
Kang, Seok Ho
author_facet Kim, Hyung Keun
Pyun, Jong Hyun
Cho, Seok
Kang, Sung Gu
Lee, Jeong Gu
Kim, Je Jong
Cheon, Jun
Park, Hong Seok
Kang, Seok Ho
author_sort Kim, Hyung Keun
collection PubMed
description PURPOSE: To evaluate tumor-specific immunity and define the mechanisms involved in the cryoimmunologic response, we compared the tumor control efficacy and immunologic responses of cryoablation with those of surgical excision in a tumor rechallenge model. MATERIALS AND METHODS: Sixty BALB/c mice with RENCA tumors that were generated in the left flank area underwent cryoablation or radical excision. The mice successfully treated were rechallenged with RENCA or an undifferentiated colon carcinoma cell line, CT26, in the contralateral right flank area. The recurrence rate after tumor rechallenge in each group was then observed. To assess the immunologic response of each treatment modality, fluorescent-activated cell sorting (FACS) analysis and a cytotoxicity assay using (51)Cr release were performed. RESULTS: After reinoculation of the RENCA cells, the rate of tumor growth was significantly higher in the surgical excision group than in the cryoablation group (94.4% vs. 11.1%, p=0.001). In the cryoablation group, the tumor growth rate was significantly increased after rechallenge of CT26 cells compared with RENCA (94.1% vs. 11.1%, p=0.001). The cryoablation group showed an elevated CD3, CD4, CD8 T, and natural killer cell count in the FACS analysis and also showed significantly increased cytotoxicity in the (51)Cr release assay compared with the excision group. CONCLUSIONS: These results showed that cryoablation, compared to surgical resection, was more effective in preventing tumor growth after rechallenge with RENCA cells and that this response was tumor-specific, because the CT26 cells did not have the same effect.
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spelling pubmed-42657192014-12-15 Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model Kim, Hyung Keun Pyun, Jong Hyun Cho, Seok Kang, Sung Gu Lee, Jeong Gu Kim, Je Jong Cheon, Jun Park, Hong Seok Kang, Seok Ho Korean J Urol Original Article PURPOSE: To evaluate tumor-specific immunity and define the mechanisms involved in the cryoimmunologic response, we compared the tumor control efficacy and immunologic responses of cryoablation with those of surgical excision in a tumor rechallenge model. MATERIALS AND METHODS: Sixty BALB/c mice with RENCA tumors that were generated in the left flank area underwent cryoablation or radical excision. The mice successfully treated were rechallenged with RENCA or an undifferentiated colon carcinoma cell line, CT26, in the contralateral right flank area. The recurrence rate after tumor rechallenge in each group was then observed. To assess the immunologic response of each treatment modality, fluorescent-activated cell sorting (FACS) analysis and a cytotoxicity assay using (51)Cr release were performed. RESULTS: After reinoculation of the RENCA cells, the rate of tumor growth was significantly higher in the surgical excision group than in the cryoablation group (94.4% vs. 11.1%, p=0.001). In the cryoablation group, the tumor growth rate was significantly increased after rechallenge of CT26 cells compared with RENCA (94.1% vs. 11.1%, p=0.001). The cryoablation group showed an elevated CD3, CD4, CD8 T, and natural killer cell count in the FACS analysis and also showed significantly increased cytotoxicity in the (51)Cr release assay compared with the excision group. CONCLUSIONS: These results showed that cryoablation, compared to surgical resection, was more effective in preventing tumor growth after rechallenge with RENCA cells and that this response was tumor-specific, because the CT26 cells did not have the same effect. The Korean Urological Association 2014-12 2014-11-28 /pmc/articles/PMC4265719/ /pubmed/25512819 http://dx.doi.org/10.4111/kju.2014.55.12.834 Text en © The Korean Urological Association, 2014 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Hyung Keun
Pyun, Jong Hyun
Cho, Seok
Kang, Sung Gu
Lee, Jeong Gu
Kim, Je Jong
Cheon, Jun
Park, Hong Seok
Kang, Seok Ho
Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title_full Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title_fullStr Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title_full_unstemmed Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title_short Tumor-Specific Immunity Induced by Cryoablation in a Murine Renal Cell Carcinoma Model
title_sort tumor-specific immunity induced by cryoablation in a murine renal cell carcinoma model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265719/
https://www.ncbi.nlm.nih.gov/pubmed/25512819
http://dx.doi.org/10.4111/kju.2014.55.12.834
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