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Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery
Understanding the effects of immune responses on targeted delivery of nanoparticles is important for clinical translations of new cancer imaging and therapeutic nanoparticles. In this study, we found that repeated administrations of magnetic iron oxide nanoparticles (IONPs) conjugated with mouse or...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265747/ https://www.ncbi.nlm.nih.gov/pubmed/25553097 http://dx.doi.org/10.7150/thno.10350 |
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author | Yang, Emmy Qian, Weiping Cao, Zehong Wang, Liya Bozeman, Erica N. Ward, Christina Yang, Bin Selvaraj, Periasamy Lipowska, Malgorzata Wang, Y. Andrew Mao, Hui Yang, Lily |
author_facet | Yang, Emmy Qian, Weiping Cao, Zehong Wang, Liya Bozeman, Erica N. Ward, Christina Yang, Bin Selvaraj, Periasamy Lipowska, Malgorzata Wang, Y. Andrew Mao, Hui Yang, Lily |
author_sort | Yang, Emmy |
collection | PubMed |
description | Understanding the effects of immune responses on targeted delivery of nanoparticles is important for clinical translations of new cancer imaging and therapeutic nanoparticles. In this study, we found that repeated administrations of magnetic iron oxide nanoparticles (IONPs) conjugated with mouse or human derived targeting ligands induced high levels of ligand specific antibody responses in normal and tumor bearing mice while injections of unconjugated mouse ligands were weakly immunogenic and induced a very low level of antibody response in mice. Mice that received intravenous injections of targeted and polyethylene glycol (PEG)-coated IONPs further increased the ligand specific antibody production due to differential uptake of PEG-coated nanoparticles by macrophages and dendritic cells. However, the production of ligand specific antibodies was markedly inhibited following systemic delivery of theranostic nanoparticles carrying a chemotherapy drug, doxorubicin. Targeted imaging and histological analysis revealed that lack of the ligand specific antibodies led to an increase in intratumoral delivery of targeted nanoparticles. Results of this study support the potential of further development of targeted theranostic nanoparticles for the treatment of human cancers. |
format | Online Article Text |
id | pubmed-4265747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-42657472015-01-01 Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery Yang, Emmy Qian, Weiping Cao, Zehong Wang, Liya Bozeman, Erica N. Ward, Christina Yang, Bin Selvaraj, Periasamy Lipowska, Malgorzata Wang, Y. Andrew Mao, Hui Yang, Lily Theranostics Research Paper Understanding the effects of immune responses on targeted delivery of nanoparticles is important for clinical translations of new cancer imaging and therapeutic nanoparticles. In this study, we found that repeated administrations of magnetic iron oxide nanoparticles (IONPs) conjugated with mouse or human derived targeting ligands induced high levels of ligand specific antibody responses in normal and tumor bearing mice while injections of unconjugated mouse ligands were weakly immunogenic and induced a very low level of antibody response in mice. Mice that received intravenous injections of targeted and polyethylene glycol (PEG)-coated IONPs further increased the ligand specific antibody production due to differential uptake of PEG-coated nanoparticles by macrophages and dendritic cells. However, the production of ligand specific antibodies was markedly inhibited following systemic delivery of theranostic nanoparticles carrying a chemotherapy drug, doxorubicin. Targeted imaging and histological analysis revealed that lack of the ligand specific antibodies led to an increase in intratumoral delivery of targeted nanoparticles. Results of this study support the potential of further development of targeted theranostic nanoparticles for the treatment of human cancers. Ivyspring International Publisher 2015-01-01 /pmc/articles/PMC4265747/ /pubmed/25553097 http://dx.doi.org/10.7150/thno.10350 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Yang, Emmy Qian, Weiping Cao, Zehong Wang, Liya Bozeman, Erica N. Ward, Christina Yang, Bin Selvaraj, Periasamy Lipowska, Malgorzata Wang, Y. Andrew Mao, Hui Yang, Lily Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title | Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title_full | Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title_fullStr | Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title_full_unstemmed | Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title_short | Theranostic Nanoparticles Carrying Doxorubicin Attenuate Targeting Ligand Specific Antibody Responses Following Systemic Delivery |
title_sort | theranostic nanoparticles carrying doxorubicin attenuate targeting ligand specific antibody responses following systemic delivery |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4265747/ https://www.ncbi.nlm.nih.gov/pubmed/25553097 http://dx.doi.org/10.7150/thno.10350 |
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